红棕象甲（Rhynchophorus ferrugineus）中气味结合蛋白基因的鉴定及组织表达谱分析
Identification and tissue expression profiling of odorant binding protein genes in the red palm weevil, Rhynchophorus ferrugineus.
作者信息
Yan Wei, Liu Li, Qin Weiquan, Luo Youqing, Ma Xuezhong, Haider Nabil, Inayeh Muhanad
机构信息
Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing, 100083 China ; Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan, 571339 China.
Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan, 571339 China.
出版信息
Springerplus. 2016 Sep 13;5(1):1542. doi: 10.1186/s40064-016-3198-x. eCollection 2016.
BACKGROUND
The red palm weevil, Rhynchophorus ferrugineus, is a lethal pest of the palms. The identification of odorant binding protein (OBP) genes will be helpful for clarifing the mechanism of odorant detection of this pest. By sequencing the full length cDNA library of its antenne, 11 OBP genes (RferOBP1-11) were identified.
FINDINGS
The result showed RferOBP1-7 and RferOBP8-11 belonged to the minus-C and classic family, respectively qPCR revealed that RferOBP1-10 highly transcribed in the antennae, of which RferOBP1, RferOBP4, RferOBP8 and RferOBP10 were obviously male-biased expression. RferOBP7 and RferOBP11 exhibited highly expression in female head and male thorax. RferOBP2, RferOBP5 and RferOBP6 were highly expressed in the female thorax, leg and abdomen respectively.
CONCLUSIONS
The results paved the way towards a future understanding of the olfaction in this species.
背景
红棕象甲（Rhynchophorus ferrugineus）是棕榈树的一种致命害虫。气味结合蛋白（OBP）基因的鉴定将有助于阐明这种害虫的气味检测机制。通过对其触角全长cDNA文库进行测序，鉴定出11个OBP基因（RferOBP1 - 11）。
研究结果
结果表明，RferOBP1 - 7和RferOBP8 - 11分别属于负C家族和经典家族。定量PCR显示，RferOBP1 - 10在触角中高转录，其中RferOBP1、RferOBP4、RferOBP8和RferOBP10表现出明显的雄性偏向性表达。RferOBP7和RferOBP11在雌性头部和雄性胸部高表达。RferOBP2、RferOBP5和RferOBP6分别在雌性胸部、腿部和腹部高表达。
结论
这些结果为未来了解该物种的嗅觉奠定了基础。
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