Shibata I, Seiki Y, Matsumoto M, Kuroki T, Terao H, Noguchi T
Department of Neurosurgery, Toho University, School of Medicine, Tokyo, Japan.
No To Shinkei. 1989 Apr;41(4):419-25.
Recently it has become evident that "second growth factor" of growth hormone (GH), such as somatomedins, has an effect on the proliferation and growth of tumor cells derived from nervous tissue. Effects of host-immunocompetence and the host-humoral states on the take incidence and proliferative activity of brain tumor cells were studied using two animal models: nude mouse and pituitary Snell dwarf mouse. Nude mouse is known to be immunodeficient. Pituitary Snell dwarf mouse is characterized by lack of circulating GH, TSH, prolactin, in addition to immunodeficiency. Cell line used in this experiment was C-6 cell of rat glioma cell. After intracranial implantation of C-6 glioma cells in the animals, the take incidence and growth rate of C-6 glioma cells were followed up and measured over a period of 2 months. Tissues of implants were studied immunohistochemically and biochemically. Regardless of cell line, successful take incidence in the different animal species was found to be greater in the descending order of nude mouse, dwarf mouse. This confirmed the role of immune status for the successful take of iso-, or heterologous tumor cells after implantation. We are now investigating the effect of exogenous GH on the growth rate of cells implanted in the dwarf mouse. This may clarify the effect of growth factors on proliferative activity of implanted tumor cells.
最近已变得明显的是，生长激素（GH）的“第二生长因子”，如生长调节素，对源自神经组织的肿瘤细胞的增殖和生长有影响。使用两种动物模型研究了宿主免疫能力和宿主体液状态对脑肿瘤细胞接种发生率和增殖活性的影响：裸鼠和垂体斯内尔侏儒小鼠。已知裸鼠免疫缺陷。垂体斯内尔侏儒小鼠的特征是除免疫缺陷外，还缺乏循环中的GH、TSH、催乳素。本实验中使用的细胞系是大鼠胶质瘤细胞C-6细胞。在动物颅内植入C-6胶质瘤细胞后，对C-6胶质瘤细胞的接种发生率和生长速率进行了2个月的随访和测量。对植入物组织进行了免疫组织化学和生化研究。无论细胞系如何，不同动物物种中成功接种的发生率按裸鼠、侏儒小鼠的顺序递减。这证实了免疫状态对植入后同种或异种肿瘤细胞成功接种的作用。我们目前正在研究外源性GH对植入侏儒小鼠体内细胞生长速率的影响。这可能会阐明生长因子对植入肿瘤细胞增殖活性的影响。
