Huang Q, Du Z W, Xu G D, Liu Z Y, Guo Y H, Chen G L, Ma W X, Tan Q Y, Xu Q N, Li B
First Hospital, Suzhou Medical College.
Zhonghua Zhong Liu Za Zhi. 1987 Jul;9(4):269-72.
Establishment and its characteristics of a nude mice solid tumor model NHG-1 from human glioma cell line are reported. 5-8 week old NC nude mice of both sexes and SHG-44 cell line used in this experiment were from our laboratory. The initial successful transplantation rate was 7/11 (64%) and that of 30 passages in the subsequent 4 years was 100%. After subcutaneous inoculation, growth curve showed a latent period in week 1-2, slow growing period in week 3-4, rapid growing period in week 5-6 and a final plateau period in week 7. The doubling time was 7 days and cell cycle time was 2.5 days. The cells in G1, S and G2M phases comprised 56%, 27% and 17%, respectively. The survival time of the host was 54 +/- 15 days. The tumor tissues showed a tendency towards invading the surrounding soft tissues. By morphological observation with light and electron microscopes, LDH isozyme assay, PAP immuno-histochemistry labelling GFAP and chromosome analysis, it is confirmed that the transplantable tumor possesses the characteristics of human malignant glioma. The estrogen receptor in the transplantable tumors demonstrated by cytochemical assay indicates that the glioma carcinogenesis is related to endocrine factor of the host. The therapeutic effects of anticancer drugs, such as ACNU, BCNU and 10-hydroxy-2-decenoic acid from the royal jelly on NHG-1 model are evaluated.
报道了源自人胶质瘤细胞系的裸鼠实体瘤模型NHG-1的建立及其特性。本实验所用的5-8周龄NC裸鼠雌雄两性以及SHG-44细胞系均来自本实验室。最初的成功移植率为7/11（64%），在随后4年的30代传代中移植率为100%。皮下接种后，生长曲线显示第1-2周为潜伏期，第3-4周为缓慢生长期，第5-6周为快速生长期，第7周为最终平台期。倍增时间为7天，细胞周期时间为2.5天。处于G1、S和G2M期的细胞分别占56%、27%和17%。宿主的存活时间为54±15天。肿瘤组织有向周围软组织浸润的趋势。通过光镜和电镜形态学观察、乳酸脱氢酶同工酶测定、PAP免疫组化标记GFAP以及染色体分析，证实可移植肿瘤具有人恶性胶质瘤的特性。细胞化学测定显示可移植肿瘤中的雌激素受体表明胶质瘤的发生与宿主的内分泌因素有关。评估了抗癌药物如ACNU、BCNU以及蜂王浆中的10-羟基-2-癸烯酸对NHG-1模型的治疗效果。
