Patterson R B, Keller J D, Silberstein E B, Kempczinski R F
Department of Surgery, University of Cincinnati Medical Center, Ohio 45267-0558.
Ann Vasc Surg. 1989 Apr;3(2):160-6. doi: 10.1016/S0890-5096(06)62010-4.
Two distinct series of experiments were performed to compare the behavior of ePTFE vascular grafts coated with basement membrane gel to that of identical grafts coated with fibronectin. Bilateral carotid interposition grafts (10 cm long) were interposed in 16 conditioned mongrel dogs. In the first series of experiments (n = 10), each graft was seeded with radiolabeled endothelial cells and initial endothelial cell adherence was determined. Following restoration of blood flow in the grafts, endothelial cell retention was measured for 24 hours. Seeding efficiency was 66.48% (+/- 13.2) for fibronectin-coated grafts and 56.58% (+/- 13.51) for gel-coated grafts. There was a slow, constant loss of activity during the first 90 minutes of imaging, and at 24 hours of observation the activity remaining on the fibronectin-coated graft was 13.2 +/- 3.98% of the initial graft activity. Although the basement membrane gel had a higher mean activity at 24 hours (18.9 +/- 7.22%), the difference was not statistically significant at any interval. In the second series of animals (n = 6), radiolabeled platelets were injected within 60 minutes following restoration of flow. Total platelet activity on the explanted grafts was 3.36 (+/- 1.35) x 10(5) counts per gram/0.2 minute for the fibronectin-coated grafts. The gel-coated grafts had 2.74 (+/- 1.33) x 10(5) counts per gram/0.2 minute, a difference that was not statistically significant. Thus, despite its theoretical appeal, basement membrane gel was no better than fibronectin in increasing endothelial cell adherence and retention, and the resulting flow surface of grafts treated with either compound appeared to attract platelets to an equal degree.
进行了两个不同系列的实验，以比较涂有基底膜凝胶的ePTFE血管移植物与涂有纤连蛋白的相同移植物的性能。在16只经过适应性训练的杂种狗中植入双侧颈动脉间置移植物（10厘米长）。在第一个系列实验（n = 10）中，每个移植物接种放射性标记的内皮细胞，并测定初始内皮细胞黏附情况。在移植物恢复血流后，测量24小时内内皮细胞的留存情况。纤连蛋白涂层移植物的接种效率为66.48%（±13.2），凝胶涂层移植物的接种效率为56.58%（±13.51）。在成像的前90分钟内，活性缓慢持续下降，在观察24小时时，纤连蛋白涂层移植物上剩余的活性为初始移植物活性的13.2±3.98%。尽管基底膜凝胶在24小时时平均活性较高（18.9±7.22%），但在任何时间间隔差异均无统计学意义。在第二个系列的动物实验（n = 6）中，在恢复血流后60分钟内注射放射性标记的血小板。对于纤连蛋白涂层移植物，取出的移植物上的总血小板活性为3.36（±1.35）×10⁵计数/克/0.2分钟。凝胶涂层移植物的总血小板活性为2.74（±1.33）×10⁵计数/克/0.2分钟，差异无统计学意义。因此，尽管基底膜凝胶在理论上有吸引力，但在增加内皮细胞黏附与留存方面并不比纤连蛋白更好，并且用这两种化合物处理的移植物所形成的血流表面似乎在吸引血小板方面程度相当。
