Abou-Shanab Reda A I, Eraky Mohamed, Haddad Ahmed M, Abdel-Gaffar Abdel-Rahman B, Salem Ahmed M
Environmental Biotechnology Department, City of Scientific Research and Technology Applications, New Borg El Arab City, Alexandria, 21934, Egypt.
Faculty of Science, Biochemistry Department, Ain Shams University, Cairo, Egypt.
Bull Environ Contam Toxicol. 2016 Nov;97(5):684-688. doi: 10.1007/s00128-016-1924-2. Epub 2016 Sep 21.
A total of twenty bacterial cultures were isolated from hydrocarbon contaminated soil. Of the 20 isolates, RAM03, RAM06, RAM13, and RAM17 were specifically chosen based on their relatively higher growth on salt medium amended with 4 % crude oil, emulsion index, surface tension, and degradation percentage. These bacterial cultures had 16S rRNA gene sequences that were most similar to Ochrobactrum cytisi (RAM03), Ochrobactrum anthropi (RAM06 and RAM17), and Sinorhizobium meliloti (RAM13) with 96 %, 100 % and 99 %, and 99 % similarity. The tested strains revealed a promising potential for bioremediation of petroleum oil contamination as they could degrade >93 % and 54 % of total petroleum hydrocarbons (TPHs) in a liquid medium and soil amended with 4 % crude oil, respectively, after 30 day incubation. These bacteria could effectively remove both aliphatic and aromatic petroleum hydrocarbons. In conclusion, these strains could be considered as good prospects for their application in bioremediation of hydrocarbon contaminated environment.
从受碳氢化合物污染的土壤中总共分离出20种细菌培养物。在这20种分离物中，RAM03、RAM06、RAM13和RAM17是根据它们在添加4%原油的盐培养基上相对较高的生长情况、乳化指数、表面张力和降解率特意挑选出来的。这些细菌培养物的16S rRNA基因序列与嗜柠檬酸慢生根瘤菌（RAM03）、人苍白杆菌（RAM06和RAM17）以及苜蓿中华根瘤菌（RAM13）最为相似，相似度分别为96%、100%、99%和99%。经过30天的培养，测试菌株在液体培养基和添加4%原油的土壤中分别能降解>93%和54%的总石油烃（TPH），显示出在生物修复石油污染方面的巨大潜力。这些细菌能够有效去除脂肪族和芳香族石油烃。总之，这些菌株有望应用于碳氢化合物污染环境的生物修复。
