Tirado-Torres David, Acevedo-Sandoval Otilio, Rodríguez-Pastrana Blanca R, Gayosso-Canales Martha
a Institute of Basic Sciences and Engineering, Autonomous University of Hidalgo (UAEH) , Pahuca, Hidalgo , Mexico.
b IInstitute of Agriculture and Livestock, Autonomous University of Hidalgo (UAEH) , Tulancingo, Hidalgo , Mexico.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2017 Jul 29;52(9):897-904. doi: 10.1080/10934529.2017.1316170. Epub 2017 May 2.
This study employed the use of 16S rRNA gene sequence analysis to identify three of four native bacterial strains isolated from crude oil-contaminated site in Poza Rica, Veracruz, Mexico. The identified bacteria were Ochrobactrum intermedium, Pandoraea pnomenusa and Ochrobactrum sp., but SA2-09 strain was not identified. The ability of the isolates to degrade polycyclic aromatic hydrocarbons (PAHs) was evaluated at 31.61 and 54.52 mg/kg PAHs in soil, when used as crude oil in soil microcosm during 80 days of incubation at 30°C. The results demonstrated that O. intermedium biodegraded many PAHs, including the high molecular weight (HMW) PAHs fluoranthene (100% equivalent 0.24 mg/kg), benzo [b] fluoranthene (81.8% equal 0.18 mg/kg), Benzo[a]pyrene (87.0%, 0.20 mg/kg) and Benzo[g,h,i]perylene (52.7%, 0.39 mg/kg). P. pnomenusa had a degradation profile of HMW PAHs, which was similar to O. intermedium, while Ochrobactrum sp. and the strain SA-09 exhibited lower degradation rates of HMW.
本研究利用16S rRNA基因序列分析，对从墨西哥韦拉克鲁斯州波萨里卡一处原油污染场地分离出的4株天然细菌菌株中的3株进行了鉴定。鉴定出的细菌为中间苍白杆菌、潘多拉菌和苍白杆菌属，但未鉴定出SA2 - 09菌株。当在30°C下培养80天的土壤微观环境中用作原油时，在土壤中PAHs含量为31.61和54.52 mg/kg的情况下，评估了这些分离菌株降解多环芳烃（PAHs）的能力。结果表明，中间苍白杆菌对许多PAHs具有生物降解作用，包括高分子量（HMW）PAHs荧蒽（100%，相当于0.24 mg/kg）、苯并[b]荧蒽（81.8%，相当于0.18 mg/kg）、苯并[a]芘（87.0%，0.20 mg/kg）和苯并[g,h,i]苝（52.7%，0.39 mg/kg）。潘多拉菌对HMW PAHs的降解情况与中间苍白杆菌相似，而苍白杆菌属和SA - 09菌株对HMW的降解率较低。
