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[1-苯胺基萘-8-磺酸盐荧光探针在低密度脂蛋白表面单层的定位]

[Localization of a 1-anilinonaphthalen-8-sulfonate fluorescent probe on the surface monolayer of low density lipoproteins].

作者信息

Omel'ianenko V G, Churakov A K, Martseniuk O V, Ruuge E K

出版信息

Biofizika. 1989 May-Jun;34(3):425-8.

PMID:2765570
Abstract

A decay of fluorescence probe 1-anilino-8-naphtalene sulfonate (ANS) sorbed on low density lipoproteins (LDL) surface obtained from human plasma was described. It was demonstrated that on the LDL surface the ANS probe is allocated among two pools of molecules with the time of fluorescence decay 4-7 ns and 12-16 ns. One may conclude that 75-90% of the probe is connected with lipid LDL matrix.

摘要

描述了吸附在从人血浆中获得的低密度脂蛋白(LDL)表面的荧光探针1-苯胺基-8-萘磺酸盐(ANS)的衰减情况。结果表明,在LDL表面,ANS探针分布在两个分子池中,荧光衰减时间分别为4 - 7纳秒和12 - 16纳秒。可以得出结论,75 - 90%的探针与LDL脂质基质相连。

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