Tverdokhlib V P, Nikonorov A A, Filippov V K, Frolov B A, Afonina S N
Nauchnye Doki Vyss Shkoly Biol Nauki. 1989(5):30-2.
The method of determination of cholesterol-7 alpha-monooxygenase activity in vivo which has a number of advantages over the existing ones is offered. [3H]cholesterin was injected into rats intravenously in the form of albumin-stabilized emulsion. In 2--4 h and then every day 3H2O radioactivity of one of the cholesterin enzymatic hydroxylation products in microsomal liver apparatus was estimated in animals' urine. The dynamics of 3H2O secretion during 7 days and dependence of the urine radioactivity upon the given radionuclide dose are shown.
提出了一种体内胆固醇-7α-单加氧酶活性的测定方法，该方法相对于现有方法具有许多优点。[3H]胆固醇以白蛋白稳定的乳剂形式静脉注射到大鼠体内。在2 - 4小时后，然后每天在动物尿液中估计微粒体肝脏器官中胆固醇酶促羟基化产物之一的3H2O放射性。显示了7天内3H2O分泌的动态以及尿液放射性对给定放射性核素剂量的依赖性。
