磷脂酰乙醇胺从层状液晶相到反相圆柱状胶束相转变的荧光去极化研究
Fluorescence depolarization study of lamellar liquid crystalline to inverted cylindrical micellar phase transition of phosphatidylethanolamine.
作者信息
Cheng K H
机构信息
Department of Physics, Texas Tech University, Lubbock 79409.
出版信息
Biophys J. 1989 Jun;55(6):1025-31. doi: 10.1016/S0006-3495(89)82901-7.
Abstract
The orientational order and rotational dynamics of 2-[3-(diphenyl-hexatrienyl) propanoyl]-3-palmitoyl-L-alpha- phosphatidylcholine (DPH-PC) embedded in dioleoylphosphatidyl-ethanolamine (DOPE) were studied by fluorescence depolarization technique. Upon increasing the temperature, the calculated wobbling diffusion constant D perpendicular of the fluorescent probe was found to decrease at the lamellar (L alpha) to inverted cylindrical (H II) phase transition (10 degrees C). This suggested that the increased gauche rotamers of the alkene chains in the HII phase imposes a constraint in the wobbling motion of the fluorophore. The calculated ratio of order parameter in the L alpha phase to that in the HII phase was 1.7 and different from the theoretical value of 2.0 as predicted from the change in packing symmetry. This result can be explained by a slightly higher local order parameter of the fluorophore or by the fast rotational diffusion motion of the fluorophore around the symmetry axis of the cylindrical tubes in the HII phase.
摘要
通过荧光去极化技术研究了嵌入二油酰磷脂酰乙醇胺（DOPE）中的2-[3-(二苯基己三烯基)丙酰基]-3-棕榈酰-L-α-磷脂酰胆碱（DPH-PC）的取向有序性和旋转动力学。随着温度升高，发现在层状（Lα）到反相圆柱状（HII）相转变（10℃）时，荧光探针的计算摆动扩散常数D⊥降低。这表明HII相中烯烃链增加的gauche旋转异构体对荧光团的摆动运动施加了限制。计算出的Lα相中的序参数与HII相中的序参数之比为1.7，不同于根据堆积对称性变化预测的理论值2.0。该结果可以通过荧光团略高的局部序参数或通过荧光团在HII相中围绕圆柱管对称轴的快速旋转扩散运动来解释。
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