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1
Fluorescence depolarization study of lamellar liquid crystalline to inverted cylindrical micellar phase transition of phosphatidylethanolamine.磷脂酰乙醇胺从层状液晶相到反相圆柱状胶束相转变的荧光去极化研究
Biophys J. 1989 Jun;55(6):1025-31. doi: 10.1016/S0006-3495(89)82901-7.
2
Effects of lateral diffusion on the fluorescence anisotropy in hexagonal lipid phases. II. An experimental study.侧向扩散对六方脂质相荧光各向异性的影响。II. 一项实验研究。
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3
Order and dynamics in the lamellar L alpha and in the hexagonal HII phase. Dioleoylphosphatidylethanolamine studied with angle-resolved fluorescence depolarization.层状Lα相和六方HII相中的有序性与动力学。用角分辨荧光去极化研究二油酰磷脂酰乙醇胺。
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4
Detection of membrane packing defects by time-resolved fluorescence depolarization.通过时间分辨荧光去极化检测膜包装缺陷
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Inverted micellar intermediates and the transitions between lamellar, cubic, and inverted hexagonal lipid phases. II. Implications for membrane-membrane interactions and membrane fusion.反胶束中间体以及层状、立方相和反六角形脂质相之间的转变。II. 对膜-膜相互作用和膜融合的影响。
Biophys J. 1986 Jun;49(6):1171-83. doi: 10.1016/S0006-3495(86)83745-6.
6
[Fluorometric analysis of liquid-crystalline to inverted hexagonal phase transition induced by diacylglycerol in liposomes].
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8
Infrared and time-resolved fluorescence spectroscopic studies of the polymorphic phase behavior of phosphatidylethanolamine/diacylglycerol lipid mixtures.磷脂酰乙醇胺/二酰基甘油脂质混合物多晶型相行为的红外和时间分辨荧光光谱研究。
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Quantitation of lateral stress in lipid layer containing nonbilayer phase preferring lipids by frequency-domain fluorescence spectroscopy.通过频域荧光光谱法定量分析含有偏好非双层相脂质的脂质层中的侧向应力。
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Fluorescence depolarization study on non-bilayer phases of phosphatidylethanolamine and phosphatidylcholine lipid mixtures.
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8
Intramolecular excimer kinetics of fluorescent dipyrenyl lipids: 2. DOPE/DOPC membranes.荧光二芘基脂质的分子内激基复合物动力学：2. 二油酰磷脂酰乙醇胺/二油酰磷脂酰胆碱膜
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Intramolecular excimer kinetics of fluorescent dipyrenyl lipids: 1. DMPC/cholesterol membranes.荧光二芘脂质的分子内激基复合物动力学：1. 二肉豆蔻酰磷脂酰胆碱/胆固醇膜
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10
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Influence of cholesterol on the structural preferences of dioleoylphosphatidylethanolamine-dioleoylphosphatidylcholine systems: a phosphorus-31 and deuterium nuclear magnetic resonance study.
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Effect of orientational order on the decay of the fluorescence anisotropy in membrane suspensions. Experimental verification on unilamellar vesicles and lipid/alpha-lactalbumin complexes.取向有序对膜悬浮液中荧光 anisotropy 衰减的影响。对单层囊泡和脂质/α-乳白蛋白复合物的实验验证。
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Effect of orientational order on the decay of the fluorescence anisotropy in membrane suspensions. A new approximate solution of the rotational diffusion equation.取向有序对膜悬浮液中荧光各向异性衰减的影响。旋转扩散方程的一种新的近似解。
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Multifrequency phase and modulation fluorometry.多频相位和调制荧光测定法。
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A differential polarized phase fluorometric study of the effects of high hydrostatic pressure upon the fluidity of cellular membranes.高静水压对细胞膜流动性影响的微分偏振相荧光测定研究。
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Advantages and limitations of 1-palmitoyl-2-[[2-[4- (6-phenyl-trans-1,3,5-hexatrienyl)phenyl]ethyl]carbonyl]-3- sn-phosphatidylcholine as a fluorescent membrane probe.1-棕榈酰-2-[[2-[4-(6-苯基反式-1,3,5-己三烯基)苯基]乙基]羰基]-3-sn-磷脂酰胆碱作为荧光膜探针的优点和局限性。
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磷脂酰乙醇胺从层状液晶相到反相圆柱状胶束相转变的荧光去极化研究

Fluorescence depolarization study of lamellar liquid crystalline to inverted cylindrical micellar phase transition of phosphatidylethanolamine.

作者信息

Cheng K H

机构信息

Department of Physics, Texas Tech University, Lubbock 79409.

出版信息

Biophys J. 1989 Jun;55(6):1025-31. doi: 10.1016/S0006-3495(89)82901-7.

DOI:10.1016/S0006-3495(89)82901-7
PMID:2765643
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1330570/
Abstract

The orientational order and rotational dynamics of 2-[3-(diphenyl-hexatrienyl) propanoyl]-3-palmitoyl-L-alpha- phosphatidylcholine (DPH-PC) embedded in dioleoylphosphatidyl-ethanolamine (DOPE) were studied by fluorescence depolarization technique. Upon increasing the temperature, the calculated wobbling diffusion constant D perpendicular of the fluorescent probe was found to decrease at the lamellar (L alpha) to inverted cylindrical (H II) phase transition (10 degrees C). This suggested that the increased gauche rotamers of the alkene chains in the HII phase imposes a constraint in the wobbling motion of the fluorophore. The calculated ratio of order parameter in the L alpha phase to that in the HII phase was 1.7 and different from the theoretical value of 2.0 as predicted from the change in packing symmetry. This result can be explained by a slightly higher local order parameter of the fluorophore or by the fast rotational diffusion motion of the fluorophore around the symmetry axis of the cylindrical tubes in the HII phase.

摘要

通过荧光去极化技术研究了嵌入二油酰磷脂酰乙醇胺（DOPE）中的2-[3-(二苯基己三烯基)丙酰基]-3-棕榈酰-L-α-磷脂酰胆碱（DPH-PC）的取向有序性和旋转动力学。随着温度升高，发现在层状（Lα）到反相圆柱状（HII）相转变（10℃）时，荧光探针的计算摆动扩散常数D⊥降低。这表明HII相中烯烃链增加的gauche旋转异构体对荧光团的摆动运动施加了限制。计算出的Lα相中的序参数与HII相中的序参数之比为1.7，不同于根据堆积对称性变化预测的理论值2.0。该结果可以通过荧光团略高的局部序参数或通过荧光团在HII相中围绕圆柱管对称轴的快速旋转扩散运动来解释。