神经节苷脂对含磷脂酰乙醇胺和二油酰甘油混合物中片层反胶束（六方Ⅱ相）相变的调节作用。
Modulation by gangliosides of the lamellar-inverted micelle (hexagonal II) phase transition in mixtures containing phosphatidylethanolamine and dioleoylglycerol.
作者信息
Perillo M A, Scarsdale N J, Yu R K, Maggio B
机构信息
Department of Biochemistry and Molecular Biophysics, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0614.
出版信息
Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):10019-23. doi: 10.1073/pnas.91.21.10019.
Abstract
We studied the effect of gangliosides GD1a and GM1 on the lamellar-to-hexagonal II phase transition of mixtures of dioleoylphosphatidylethanolamine/dioleoylphosphatidyl choline, 3:1, and of transphosphatidylated phosphatidylethanolamine with dioleoylglycerol by high-sensitivity differential scanning calorimetry, 31P-NMR, and pyrene fluorescence of a phosphatidylcholine probe. Gangliosides had a dual effect. Below 1 mol % ganglioside the hexagonal II phase transition was affected but still occurred at lower temperature than in the absence of gangliosides. The presence of between 1 and 2 mol % gangliosides increased the temperature for formation of the hexagonal II phase and progressively decreased its cooperativity. Above 3 mol % gangliosides totally inhibited the formation of both the temperature-induced and composition-induced hexagonal phase, probably by opposing the geometric distortions necessary for the inverted micellar structures.
摘要
我们通过高灵敏度差示扫描量热法、³¹P-NMR以及磷脂酰胆碱探针的芘荧光法，研究了神经节苷脂GD1a和GM1对3:1的二油酰磷脂酰乙醇胺/二油酰磷脂酰胆碱混合物以及转磷脂酰化磷脂酰乙醇胺与二油酰甘油混合物的片层-六方II相转变的影响。神经节苷脂具有双重作用。低于1 mol%的神经节苷脂时，六方II相转变受到影响，但仍在比不存在神经节苷脂时更低的温度下发生。1至2 mol%的神经节苷脂的存在提高了六方II相形成的温度，并逐渐降低其协同性。高于3 mol%的神经节苷脂可能通过对抗反胶束结构所需的几何畸变，完全抑制了温度诱导和组成诱导的六方相的形成。
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