通过荧光寿命异质性检测脂质双层中的临界密度涨落
Critical density fluctuations in lipid bilayers detected by fluorescence lifetime heterogeneity.
作者信息
Ruggiero A, Hudson B
机构信息
Department of Chemistry, University of Oregon, Eugene 97403.
出版信息
Biophys J. 1989 Jun;55(6):1111-24. doi: 10.1016/S0006-3495(89)82908-X.
Abstract
The heterogeneity of the decay of the fluorescence of transparinaric acid in single-component lipid bilayers at temperatures above their gel/liquid crystalline phase transition is shown to be due to the presence of regions of higher local density and higher acyl chain order than the predominant fluid regions. This conclusion is based on selective excitation behavior and the observation of time-resolved fluorescence anisotropies that increase at long times. The fractional amplitude of the long lifetime component of the fluorescence shows a temperature variation that conforms to conventional descriptions of critical behavior. The critical exponent extracted from this variation is 1.1, close to the value of 1.0 that describes ultrasonic data. We therefore conclude that liquid crystalline lipid bilayers exhibit critical behavior with significant density and order fluctuations. This behavior must be taken into account in the interpretation of fluorescence and other spectroscopic measurements of the properties of bilayers.
摘要
在高于其凝胶/液晶相转变温度的单组分脂质双层中，透明质酸荧光衰减的异质性被证明是由于存在比主要流体区域具有更高局部密度和更高酰基链有序度的区域。这一结论基于选择性激发行为以及对长时间增加的时间分辨荧光各向异性的观察。荧光长寿命组分的分数振幅显示出符合临界行为传统描述的温度变化。从这种变化中提取的临界指数为1.1，接近描述超声数据的1.0值。因此，我们得出结论，液晶脂质双层表现出具有显著密度和有序波动的临界行为。在解释双层性质的荧光和其他光谱测量时，必须考虑这种行为。
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本文引用的文献
1
A theory of the effects of head-group structure and chain unsaturation on the chain melting transition of phospholipid dispersions.关于头部基团结构和链不饱和度对磷脂分散体链熔化转变影响的理论
Biochemistry. 1980 Sep 2;19(18):4279-93. doi: 10.1021/bi00559a021.
2
3
Critical effects from lipid-protein interaction in membranes. I. Theoretical description.膜中脂-蛋白相互作用的关键效应。I. 理论描述。
Biophys J. 1981 Nov;36(2):329-45. doi: 10.1016/S0006-3495(81)84735-2.
4
Bilayer acyl chain dynamics and lipid-protein interaction: the effect of the M13 bacteriophage coat protein on the decay of the fluorescence anisotropy of parinaric acid.双层酰基链动力学与脂-蛋白相互作用：M13噬菌体外壳蛋白对十八碳四烯酸荧光各向异性衰减的影响。
Biophys J. 1982 Jan;37(1):253-62. doi: 10.1016/S0006-3495(82)84674-2.
5
Anomalies of nanosecond ultrasonic relaxation in the lipid bilayer transition.脂质双层转变中纳秒级超声弛豫的异常现象。
Biochim Biophys Acta. 1982 Jun 14;688(2):411-21. doi: 10.1016/0005-2736(82)90352-2.
6
Ultrasonic measurements of two-component lipid bilayer suspensions.
Biophys Chem. 1981 Oct;14(2):147-58. doi: 10.1016/0301-4622(81)85015-6.
7
Fluorescence lifetime and time-resolved polarization anisotropy studies of acyl chain order and dynamics in lipid bilayers.脂质双层中酰基链有序性和动力学的荧光寿命及时间分辨偏振各向异性研究。
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8
Melittin induces fusion of unilamellar phospholipid vesicles.蜂毒肽可诱导单层磷脂囊泡融合。
Biochim Biophys Acta. 1983 Aug 10;732(3):668-74. doi: 10.1016/0005-2736(83)90245-6.
9
Lipid domains in membranes.膜中的脂质结构域。
Ann N Y Acad Sci. 1982;401:61-75. doi: 10.1111/j.1749-6632.1982.tb25707.x.
10
Analysis of time-resolved fluorescence anisotropy decays.时间分辨荧光各向异性衰减分析
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