时间分辨荧光各向异性衰减分析
Analysis of time-resolved fluorescence anisotropy decays.
作者信息
Cross A J, Fleming G R
出版信息
Biophys J. 1984 Jul;46(1):45-56. doi: 10.1016/S0006-3495(84)83997-1.
Abstract
We discuss the analysis of time-correlated single photon counting measurements of fluorescence anisotropy. Particular attention was paid to the statistical properties of the data. The methods used previously to analyze these experiments were examined and a new method was proposed in which parallel- and perpendicular-polarized fluorescence curves were fit simultaneously. The new method takes full advantage of the statistical properties of the measured curves; and, in some cases, it is shown to be more sensitive than other methods to systematic errors present in the data. Examples were presented using experimental and simulated data. The influence of fitting range on extracted parameters and statistical criteria for evaluating the quality of fits are also discussed.
摘要
我们讨论了荧光各向异性的时间相关单光子计数测量分析。特别关注了数据的统计特性。研究了先前用于分析这些实验的方法，并提出了一种新方法，其中同时拟合平行和垂直偏振荧光曲线。新方法充分利用了测量曲线的统计特性；并且在某些情况下，它被证明比其他方法对数据中存在的系统误差更敏感。使用实验数据和模拟数据给出了示例。还讨论了拟合范围对提取参数的影响以及评估拟合质量的统计标准。
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