高效液相色谱-荧光检测法快速灵敏测定动物脑中的β-苯乙胺
Rapid and sensitive determination of beta-phenylethylamine in animal brains by high performance liquid chromatography with fluorometric detection.
作者信息
Taga C, Tsuji M, Nakajima T
机构信息
Department of Neuropsychiatry, Kyoto Prefectural University of Medicine, Japan.
出版信息
Biomed Chromatogr. 1989 May;3(3):118-20. doi: 10.1002/bmc.1130030306.
A reversed phase HPLC method with fluorometric detection for the analysis of beta-phenylethylamine has been developed using p-methoxyphenylethylamine as an internal standard. Two columns, containing 200 microL of Dowex 50-X8 and Amberlite CG-50 respectively, were used to prepare a fraction containing beta-phenylethylamine. The recoveries of beta-phenylethylamine and p-methoxyphenylethylamine were 53.9 +/- 9.4% and 68.1 +/- 12.4%, respectively, and elution profile of p-methoxyphenylethylamine was sufficiently well correlated with that of beta-phenylethylamine. Regional distributions of beta-phenylethylamine in rat and mouse brains were determined. The highest concentrations were found in hypothalamus and hippocampus in both animals.
已开发出一种采用荧光检测的反相高效液相色谱法，以对甲氧基苯乙胺为内标分析β-苯乙胺。使用分别含有200微升Dowex 50-X8和Amberlite CG-50的两根柱来制备含β-苯乙胺的馏分。β-苯乙胺和对甲氧基苯乙胺的回收率分别为53.9±9.4%和68.1±12.4%，且对甲氧基苯乙胺的洗脱曲线与β-苯乙胺的洗脱曲线有足够好的相关性。测定了大鼠和小鼠脑中β-苯乙胺的区域分布。在两种动物的下丘脑和海马体中均发现了最高浓度。
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