McHenry Jenna A, Robison Christopher L, Bell Genevieve A, Vialou Vincent V, Bolaños-Guzmán Carlos A, Nestler Eric J, Hull Elaine M
Department of Psychology, Florida State University.
Department of Neuroscience, Icahn School of Medicine at Mount Sinai.
Behav Neurosci. 2016 Oct;130(5):469-78. doi: 10.1037/bne0000160.
The transcription factor deltaFosB (ΔFosB) is induced in the nucleus accumbens (NAc) by repeated exposure to drugs of abuse and natural rewards. Less is known about its role in other brain areas. Here, we compared the effects of mating versus cocaine history on induction of ΔFosB in the medial preoptic area (MPOA), an integral site for reproductive behavior, and in the NAc. ΔFosB immunoreactivity (ir) was increased in the MPOA of previously naïve and experienced male rats that mated the day before euthanasia, compared to unmated controls and experienced males with recent mating abstinence. Western immunoblots confirmed that the 35-37-kDa isoform of ΔFosB was increased more in recently mated males. Conversely, previous plus recent cocaine did not increase ΔFosB-ir in the MPOA, despite an increase in the NAc. Next, a viral vector expressing ΔFosB, its dominant negative antagonist ΔJunD, or green fluorescent protein (GFP) control, were microinjected bilaterally into the MPOA. ΔFosB overexpression impaired copulation and promoted female-directed aggression, compared to ΔJunD and control males. These data suggest that ΔFosB in the mPOA is expressed in an experience-dependent manner and affects systems that coordinate mating and aggression. (PsycINFO Database Record
转录因子δFosB（ΔFosB）在伏隔核（NAc）中由反复接触滥用药物和自然奖赏所诱导。关于其在其他脑区的作用了解较少。在此，我们比较了交配经历与可卡因使用史对内侧视前区（MPOA，生殖行为的一个重要部位）和伏隔核中ΔFosB诱导的影响。与未交配的对照和近期有交配禁欲的有经验雄性大鼠相比，在安乐死当天交配过的初配和有经验雄性大鼠的内侧视前区中，ΔFosB免疫反应性（ir）增加。蛋白质免疫印迹法证实，在近期交配过的雄性大鼠中，35 - 37 kDa的ΔFosB同工型增加得更多。相反，既往加近期使用可卡因并未增加内侧视前区的ΔFosB - ir，尽管伏隔核中有增加。接下来，将表达ΔFosB、其显性负性拮抗剂ΔJunD或绿色荧光蛋白（GFP）对照的病毒载体双侧微量注射到内侧视前区。与注射ΔJunD和对照的雄性大鼠相比，ΔFosB过表达损害了交配行为并促进了对雌性的攻击行为。这些数据表明，内侧视前区中的ΔFosB以经验依赖的方式表达，并影响协调交配和攻击行为的系统。（PsycINFO数据库记录）
