Ghorbani Ahmad, Mojarrad Majid, Hatami Ali, Hoseini Seyed Javad, Ghazavi Hamed, Hosseini Azar
Pharmacological Research Center of Medicinal Plants, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Exp Clin Endocrinol Diabetes. 2017 Jan;125(1):33-41. doi: 10.1055/s-0042-113460. Epub 2016 Sep 22.
Accumulated evidence indicates that there are intrinsic differences between adipose tissue-derived stem cells (ASCs) obtained from different body fat depots. Here, we compared the proliferation and multipotency of subcutaneous ASCs (SC-ASCs) and epididymal ASCs (ED-ASCs) before and after induction of diabetes by streptozotocin. The adipogenic and osteogenic abilities of rat SC-ASCs and ED-ASCs were evaluated using Oil Red O and Alizarin Red staining, respectively. The expression of adipocyte (PPAR-γ, LPL) and osteoblast (ALP, SPP1) specific mRNAs was evaluated by quantitative real-time PCR. MTT test was used for determination of cell proliferation capacity. The proliferation of SC-ASCs was higher than ED-ASCs, both before and after diabetes induction (P<0.05). Diabetes increased the proliferative capability of SC-ASCs (P<0.05) but not ED-ASCs. Before diabetes, both adipogenic and osteogenic differentiation of SC-ASCs were higher than ED-ASCs (P<0.05). After diabetes, both SC-ASCs and ED-ASCs were able to differentiate into adipocyte and osteoblast, but the levels of differentiation were higher in SC-ASCs than in ED-ASCs (P<0.05). Diabetes decreased the expression of PPAR-γ and LPL, but increased the SPP1 and ALP expression in both SC-ASCs and ED-ASCs. Our data suggested that diabetes increases the proliferation of ASCs but decreases their adipogenic differentiation. Also, SC-ASCs have higher proliferation and differentiation abilities than ED-ASCs in normal and diabetic conditions so can be more preferable for cell therapy.
越来越多的证据表明，从不同体脂储存部位获取的脂肪组织来源干细胞（ASC）存在内在差异。在此，我们比较了链脲佐菌素诱导糖尿病前后皮下ASC（SC-ASC）和附睾ASC（ED-ASC）的增殖能力和多能性。分别使用油红O和茜素红染色评估大鼠SC-ASC和ED-ASC的成脂和成骨能力。通过定量实时PCR评估脂肪细胞（PPAR-γ、LPL）和成骨细胞（ALP、SPP1）特异性mRNA的表达。采用MTT试验测定细胞增殖能力。糖尿病诱导前后，SC-ASC的增殖均高于ED-ASC（P<0.05）。糖尿病增加了SC-ASC的增殖能力（P<0.05），但未增加ED-ASC的增殖能力。糖尿病前，SC-ASC的成脂和成骨分化均高于ED-ASC（P<0.05）。糖尿病后，SC-ASC和ED-ASC均能分化为脂肪细胞和成骨细胞，但SC-ASC的分化水平高于ED-ASC（P<0.05）。糖尿病降低了SC-ASC和ED-ASC中PPAR-γ和LPL的表达，但增加了SPP1和ALP的表达。我们的数据表明，糖尿病增加了ASC的增殖，但降低了它们的成脂分化。此外，在正常和糖尿病条件下，SC-ASC比ED-ASC具有更高的增殖和分化能力，因此可能更适合用于细胞治疗。
