Assem Mostafa, Kamal Samia, Sabry Dina, Soliman Nadia, Aly Riham M
National Research Centre, Cairo, Egypt.
Cairo University Faculty of Oral and Dental Medicine, Cairo, Egypt.
Open Access Maced J Med Sci. 2018 Feb 1;6(2):254-259. doi: 10.3889/oamjms.2018.076. eCollection 2018 Feb 15.
Stem cells have recently received great interest as potential therapeutics alternative for a variety of diseases. The oral and maxillofacial region, in particular, encompasses a variety of distinctive mesenchymal (MSC) populations and is characterized by a potent multilineage differentiation capacity.
In this report, we aimed to investigate the effect of diabetes on the proliferation potential of stem cells isolated from controlled diabetic patients (type 2) and healthy individuals.
SUBJECTS & METHODS: The proliferation rate of gingival and periodontal derived stem cells isolated from diabetic & healthy individuals were compared using MTT Assay. Expression levels of Survivin in isolated stem cells from all groups were measured by qRt - PCR.
There was a significantly positive correlation between proliferation rate and expression of Survivin in all groups which sheds light on the importance of Survivin as a reliable indicator of proliferation. The expression of Survivin further confirmed the proliferation results from MTT Assay where the expression of stem cells from non - diabetic individuals was higher than diabetic patients.
Taking together all the results, it could be concluded that PDLSC and GSC are promising candidates for autologous regenerative therapy due to their ease of accessibility in addition to their high proliferative rates.
干细胞作为多种疾病潜在的治疗替代方法，近来备受关注。尤其是口腔颌面部包含多种独特的间充质干细胞（MSC）群体，且具有强大的多向分化能力。
在本报告中，我们旨在研究糖尿病对从2型糖尿病患者和健康个体中分离出的干细胞增殖潜能的影响。
使用MTT法比较从糖尿病患者和健康个体中分离出的牙龈和牙周来源干细胞的增殖率。通过qRt - PCR检测所有组分离出的干细胞中Survivin的表达水平。
所有组的增殖率与Survivin表达之间存在显著正相关，这揭示了Survivin作为增殖可靠指标的重要性。Survivin的表达进一步证实了MTT法的增殖结果，即非糖尿病个体的干细胞表达高于糖尿病患者。
综合所有结果，可以得出结论，牙周膜干细胞（PDLSC）和牙龈干细胞（GSC）因其易于获取且增殖率高，是自体再生治疗的有前途的候选者。
