Hang Xiaofeng, Wu Zhiqin, Chu Kaijian, Yu Guanzhen, Peng Haoran, Xin Haiguang, Miao Xiaohui, Wang Junxue, Xu Wensheng
Department of Infection Diseases, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, 200003, Shanghai, People's Republic of China.
Department VI of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, 200433, Shanghai, People's Republic of China.
Tumour Biol. 2016 Nov;37(11):15079-15085. doi: 10.1007/s13277-016-5302-9. Epub 2016 Sep 22.
The aim of this study was to investigate the prognostic value of dicarbonyl/L-xylulose reductase (DCXR) in human hepatocellular carcinoma (HCC). Immunohistochemistry and tissue microarrays were used to evaluate DCXR protein expression levels. Image-Pro Plus was used to calculate the integral optic density (IOD) in each tissue sample, which represented the expression level of DCXR. DCXR proteins were found to be significantly lower in HCC tumor tissues (P < 0.0001) according to immunohistochemical analysis of DCXR protein levels in 74 paired HCC tissue and peritumoral non-cancer tissues. The prognostic value of DCXR in HCC was assessed in 290 cases of the training cohort and 74 cases of the validation cohort. Shorter overall survival (OS) time and shorter time to recurrence (TTR) in both the training and validation set were found to be associated with lower expression levels of DCXR. In the training set, the expression level of DCXR in HCC was an independent prognostic factor for OS according to univariate and multivariate analyses. In conclusion, DCXR expression is an independent prognostic factor for OS and TTR of post-operative HCC patients, and low expression levels of DCXR in HCC may indicate poor outcome of HCC patients after surgical resection.
本研究旨在探讨二羰基/L-木酮糖还原酶（DCXR）在人类肝细胞癌（HCC）中的预后价值。采用免疫组织化学和组织芯片评估DCXR蛋白表达水平。使用Image-Pro Plus计算每个组织样本中的积分光密度（IOD），其代表DCXR的表达水平。根据对74对HCC组织和癌旁非癌组织中DCXR蛋白水平的免疫组织化学分析，发现HCC肿瘤组织中的DCXR蛋白显著降低（P < 0.0001）。在290例训练队列和74例验证队列中评估了DCXR在HCC中的预后价值。发现在训练集和验证集中，较短的总生存期（OS）和较短的复发时间（TTR）均与DCXR的低表达水平相关。在训练集中，根据单因素和多因素分析，HCC中DCXR的表达水平是OS的独立预后因素。总之，DCXR表达是术后HCC患者OS和TTR的独立预后因素，HCC中DCXR的低表达水平可能表明HCC患者手术切除后的预后不良。
