Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China.
Shenyang Academy of Environmental Sciences, Shenyang 110167, China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Bioresour Technol. 2016 Dec;221:666-670. doi: 10.1016/j.biortech.2016.09.047. Epub 2016 Sep 13.
Chloramphenicol (CAP) is a frequently detected environmental pollutant. In this study, an electroactive biofilm for CAP reduction was established by initially in the anode and then inverting to the cathode. The established biocathode could enhance the reduction of CAP to the nitro-group reduced CAP (AMCl2) and further dechlorinated form (AMCl), both had lost the antibacterial activity. The phylogenetic diversity of the acclimated biofilm was decreased after the polar inversion. Proportions of functional bacterial genera, including Geobacter, Desulfovibrio and Pseudomonas responsible for the bidirectional electron transfer and nitroaromatics reduction, had increased 28%, 104% and 43% in the cathode. The relatively high abundances (over 50%) of Geobacter in anode and cathode were rarely detected for the nitroaromatics reduction. This study provides new insights into the electroactive biofilm structure improvement by the polarity inversion strategy for refractory antibiotics degradation.
氯霉素（CAP）是一种常见的环境污染物。在这项研究中，通过先在阳极再反转到阴极的方式，建立了一种用于 CAP 还原的电活性生物膜。建立的生物阴极可以促进 CAP 的还原，生成失去抗菌活性的硝基还原 CAP（AMCl2）和进一步脱氯形式（AMCl）。极性反转后，驯化生物膜的系统发育多样性降低。负责双向电子转移和硝基芳烃还原的功能细菌属，包括 Geobacter、Desulfovibrio 和 Pseudomonas 的比例在阴极分别增加了 28%、104%和 43%。阳极和阴极中 Geobacter 的相对高丰度（超过 50%）很少被检测到用于硝基芳烃的还原。这项研究为通过极性反转策略改善电活性生物膜结构以降解难处理抗生素提供了新的见解。
