Deng Yuan-Fan, Liu Yuan-Yuan, Zhang Yi-Tao, Wang Yan, Liang Juan-Boo, Tufarelli Vincenzo, Laudadio Vito, Liao Xin-Di
College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Key Laboratory of Tropical Agricultural Environment, South China Agricultural University, Guangzhou, China.
J Sci Food Agric. 2017 Jun;97(8):2382-2391. doi: 10.1002/jsfa.8050. Epub 2016 Oct 18.
The efficacy and role of inulin in the mitigation of enteric sulfur-containing odor gases hydrogen sulfide (H S) and methyl mercaptan (CH SH) in pigs were examined in this study. Twelve Duroc × Landrace × Yorkshire male finisher pigs (60.7 ± 1.9 kg), housed individually in open-circuit respiration chambers, were randomly assigned to two dietary groups, namely basal diet (control) and basal diet supplemented with 1% (w/w) inulin. At the end of the 45 day experiment, pigs were slaughtered and volatile fatty acid (VFA) concentration, sulfate radical (SO ) concentration, population of sulfate-reducing bacteria (SRB) and expression of methionine gamma-lyase (MGL) gene were determined in contents from the caecum, colon (two segments) and rectum. Metabonomic analysis was used to compare differences in biochemical composition, and the Illumina MiSeq procedure to investigate differences in bacterial components, in the different parts of the large intestine between inulin-supplemented and inulin-free (control) groups.
Inulin decreased (P < 0.05) the average daily enteric H S and CH SH production by 12.4 and 12.1% respectively. The concentrations of acetate, propionate and butyrate in the large intestinal content were significantly increased (P < 0.05) with inulin treatment, whereas valerate concentration and MGL mRNA expression decreased (P < 0.05). The growth of Lactobacillus, Butyrivibrio, Pseudobutyrivibrio, Bifidobacterium and Clostridium butyricum was stimulated, while that of Desulfovibrio, the dominant SRB, was inhibited, and there was an accumulation of SO in the large intestinal content of the inulin-supplemented pigs, suggesting that inulin mitigates H S generation from the SO reduction pathway by reducing the growth of SRB.
The results showed that inulin mitigates CH SH generation via three methionine degradation metabolic pathways and H S generation from two cysteine degradation metabolic pathways, thus resulting in increased synthesis of these two sulfur-containing amino acids in the pig large intestine. © 2016 Society of Chemical Industry.
本研究检测了菊粉在减轻猪肠道含硫气味气体硫化氢（H₂S）和甲硫醇（CH₃SH）方面的功效及作用。12头杜洛克×长白×约克夏雄性育肥猪（体重60.7±1.9千克），单独饲养于开路呼吸室中，随机分为两个日粮组，即基础日粮（对照组）和添加1%（w/w）菊粉的基础日粮组。在45天实验结束时，屠宰猪并测定盲肠、结肠（两段）和直肠内容物中挥发性脂肪酸（VFA）浓度、硫酸根（SO₄²⁻）浓度、硫酸盐还原菌（SRB）数量及甲硫氨酸γ-裂合酶（MGL）基因的表达。采用代谢组学分析比较生化组成差异，利用Illumina MiSeq程序研究添加菊粉组和无菊粉（对照）组大肠不同部位细菌组成的差异。
菊粉使每日肠道H₂S和CH₃SH平均产量分别降低了12.4%和12.1%（P<0.05）。菊粉处理使大肠内容物中乙酸、丙酸和丁酸浓度显著升高（P<0.05），而戊酸浓度和MGL mRNA表达降低（P<）。刺激了乳酸杆菌、丁酸弧菌、假丁酸弧菌、双歧杆菌和丁酸梭菌的生长，同时抑制了优势SRB脱硫弧菌的生长，且添加菊粉的猪大肠内容物中SO₄²⁻有积累，表明菊粉通过减少SRB的生长减轻了SO₄²⁻还原途径中H₂S的生成。
结果表明，菊粉通过三种甲硫氨酸降解代谢途径减轻CH₃SH的生成，并通过两种半胱氨酸降解代谢途径减轻H₂S的生成，从而导致猪大肠中这两种含硫氨基酸的合成增加。©2016化学工业协会。
