Bordin K, Saladino F, Fernández-Blanco C, Ruiz M J, Mañes J, Fernández-Franzón M, Meca G, Luciano F B
School of Life Sciences, Pontifícia Universidade Católica, Rua Imaculada Conceição 1155, 80215-910 Curitiba, Paraná, Brazil.
Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain.
Food Chem. 2017 Feb 15;217:648-654. doi: 10.1016/j.foodchem.2016.09.044. Epub 2016 Sep 9.
This study investigates the reduction of zearalenone (ZEA) and α-zearalenol (α-ZOL) on a solution model using allyl isothiocyanate (AITC) and also determines the bioaccessibility and bioavailability of the reaction products isolated and identified by MS-LIT. Mycotoxin reductions were dose-dependent, and ZEA levels decreased more than α-ZOL, ranging from 0.2 to 96.9% and 0 to 89.5% respectively, with no difference (p⩽0.05) between pH 4 and 7. Overall, simulated gastric bioaccessibility was higher than duodenal bioaccessibility for both mycotoxins and mycotoxin-AITC conjugates, with duodenal fractions representing ⩾63.5% of the original concentration. Simulated bioavailability of reaction products (α-ZOL/ZEA-AITC) were lower than 42.13%, but significantly higher than the original mycotoxins. The cytotoxicity of α-ZOL and ZEA in Caco-2/TC7 cells was also evaluated, with toxic effects observed at higher levels than 75μM. Further studies should be performed to evaluate the toxicity and estrogenic effect of α-ZOL/ZEA-AITC.
本研究使用异硫氰酸烯丙酯（AITC）在溶液模型中研究了玉米赤霉烯酮（ZEA）和α-玉米赤霉醇（α-ZOL）的降解情况，并确定了通过质谱-激光诱导热解（MS-LIT）分离和鉴定的反应产物的生物可及性和生物利用度。霉菌毒素的降解呈剂量依赖性，ZEA水平的下降幅度大于α-ZOL，分别为 $0.2%$ 至 $96.9%$ 和 $0%$ 至 $89.5%$，在pH值为4和7时无差异（$p⩽0.05$）。总体而言，对于两种霉菌毒素及其与AITC的共轭物，模拟胃液中的生物可及性均高于十二指肠中的生物可及性，十二指肠部分占原始浓度的 $⩾63.5%$。反应产物（α-ZOL/ZEA-AITC）的模拟生物利用度低于 $42.13%$，但显著高于原始霉菌毒素。还评估了α-ZOL和ZEA在Caco-2/TC7细胞中的细胞毒性，在高于 $75μM$ 的水平观察到毒性作用。应进行进一步研究以评估α-ZOL/ZEA-AITC的毒性和雌激素效应。
