Li Zhijian, Wang Peng, Jiang Chengyan, Cui Pengfei, Zhang Shicui
Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266003, China.
Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266003, China; College of Life Science and Technology, Hong He University, Mengzi, Yunnan 661100, China.
Fish Shellfish Immunol. 2016 Nov;58:370-379. doi: 10.1016/j.fsi.2016.09.044. Epub 2016 Sep 22.
Pt5e, a mutant peptide derived from the C-terminal 55 residues of zebrafish phosvitin, has been suggested to be a novel antibacterial peptide. However, if it is applicable to clinical MDR bacteria remains to be tested. In this study, high-purity Pt5e was first expressed and purified by fusion with cationic elastin-like polypeptide. Pt5e was then shown to be capable of effectively killing all the five clinical MDR bacteria tested. Pt5e kill the MDR bacteria at several levels, including inserting into the bacterial membranes, causing the membrane depolarization and permeabilization, and inducing the intracellular apoptosis/necrosis. All these data suggest that Pt5e is a promising therapeutic potential as an antibiotics against clinical MDR bacteria.
Pt5e是一种源自斑马鱼卵黄高磷蛋白C末端55个残基的突变肽，已被认为是一种新型抗菌肽。然而，它是否适用于临床多重耐药菌仍有待测试。在本研究中，首先通过与阳离子弹性蛋白样多肽融合表达并纯化了高纯度的Pt5e。然后发现Pt5e能够有效杀灭所测试的全部五种临床多重耐药菌。Pt5e在多个层面杀死多重耐药菌，包括插入细菌细胞膜、导致膜去极化和通透性增加，以及诱导细胞内凋亡/坏死。所有这些数据表明，Pt5e作为一种抗临床多重耐药菌的抗生素具有有前景的治疗潜力。
