Kang Tae Hwa, Han Sang Hoon, Park Sun Jae
Animal Resources Division, National Institute of Biological Resources, 42, Hwangyeong-ro, Seo-gu, Incheon 22689, Korea.
Department of Life Science, College of Natural Science, Kyonggi University, 154-42, Gwanggyosan-ro, Yeongtong-gu, Suwon 16227, Korea.
Int J Mol Sci. 2016 Sep 23;17(10):1621. doi: 10.3390/ijms17101621.
In the present study, we used next-generation sequencing to develop 12 novel microsatellite markers for genetic structural analysis of Dorcus titanus castanicolor (Lucanidae; Coleoptera), a popular pet insect in China, Korea, and Japan. We identified 52,357 microsatellite loci in 339,287,381 bp of genomic sequence and selected 19 of the loci based on their PCR amplification efficiency and polymorphism. The 19 selected markers were then tested for the presence of null alleles and linkage disequilibrium. We did not detect any evidence of null alleles; however, four pairs of loci (DT03 and DT11, DT05 and DT26, DT08 and DT26, DT26 and DT35) exhibited linkage disequilibrium. Thus, we assessed the genetic diversity of a D. titanus castanicolor population from the Daejeon region of Korea (n = 22) using 13 markers. Among them, one marker (DT17) deviated from Hardy-Weinberg equilibrium. Therefore, 12 markers may be useful for further analyzing the genetic diversity of D. titanus castanicolor.
在本研究中，我们使用下一代测序技术开发了12个新型微卫星标记，用于对中国、韩国和日本流行的宠物昆虫——深山锹甲（Lucanidae；鞘翅目）的遗传结构进行分析。我们在339,287,381 bp的基因组序列中鉴定出52,357个微卫星位点，并根据其PCR扩增效率和多态性选择了19个位点。然后对这19个选定的标记进行无效等位基因和连锁不平衡检测。我们未检测到任何无效等位基因的证据；然而，四对位点（DT03和DT11、DT05和DT26、DT08和DT26、DT26和DT35）表现出连锁不平衡。因此，我们使用13个标记评估了来自韩国大田地区的深山锹甲种群（n = 22）的遗传多样性。其中，一个标记（DT17）偏离了哈迪-温伯格平衡。因此，12个标记可能有助于进一步分析深山锹甲的遗传多样性。
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