Waikham Pimubon, Thongkumkoon Patcharawadee, Chomdej Siriwadee, Liu Aizhong, Wangpakapattanawong Prasit
Department of Biology, Faculty of Science, Chiang Mai University, 239, HuayKaew Road, Muang District, Chiang Mai, 50200, Thailand.
Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, 132# Lanhei Road, Heilongtan, Kunming, 650201, Yunnan, P.R. China.
Mol Biol Rep. 2018 Feb;45(1):27-30. doi: 10.1007/s11033-017-4137-9. Epub 2017 Dec 20.
Catanopsis tribuloides is a climax tree species commonly distributed in evergreen forests and has been used to restore degraded areas in northern Thailand. To aid in study of genetic diversity of the species, microsatellite markers, which are specific to C. tribuloides, were developed using whole genome sequencing by next-generation sequencing technology. The primers for microsatellite were developed and screened for optimal annealing temperature by PCR assay. The loci primers specific with C. tribuloides, 13 polymorphic microsatellite primers were successfully developed. The results from genetic information analyzing showed the number of alleles presented were between 2 and 24. Accordingly, the expected and observed heterozygosity obtained were between 0.298 and 0.920 and 0.364 to 1.000, respectively. Null allele frequency was presented 0.000-0.199. Genetic information was generated 10 loci primers significantly deviated from Hardy-Weinberg Equilibrium. All 13 primer pairs of loci were not significant with linkage disequilibrium. A set of microsatellite markers in this study could be applied to gene flow, genetic structure and population genetic studies in the future.
刺蒴栲是一种常见于常绿森林的顶级树种，已被用于泰国北部退化地区的恢复。为了有助于对该物种的遗传多样性进行研究，利用下一代测序技术通过全基因组测序开发了刺蒴栲特有的微卫星标记。开发了微卫星引物，并通过PCR分析筛选出最佳退火温度。成功开发了13个与刺蒴栲特异的多态性微卫星引物位点引物。遗传信息分析结果显示，呈现的等位基因数在2至24之间。相应地，获得的预期杂合度和观察杂合度分别在0.298至0.920和0.364至1.000之间。无效等位基因频率为0.000 - 0.199。遗传信息显示10个位点引物显著偏离哈迪-温伯格平衡。所有13对位点引物均无显著连锁不平衡。本研究中的一组微卫星标记未来可应用于基因流、遗传结构和群体遗传学研究。
