DRG2通过细胞周期蛋白B1-细胞周期蛋白依赖性激酶1复合物调控G2/M期进程。
DRG2 Regulates G2/M Progression via the Cyclin B1-Cdk1 Complex.
作者信息
Jang Soo Hwa, Kim Ah-Ram, Park Neung-Hwa, Park Jeong Woo, Han In-Seob
机构信息
School of Biological Sciences, University of Ulsan, Ulsan 44610, Korea.
Department of Internal Medicine, University of Ulsan College of Medicine, Ulsan University Hospital, Ulsan 44033, Korea.
出版信息
Mol Cells. 2016 Sep;39(9):699-704. doi: 10.14348/molcells.2016.0149. Epub 2016 Sep 27.
Developmentally regulated GTP-binding protein 2 (DRG2) plays an important role in cell growth. Here we explored the linkage between DRG2 and G2/M phase checkpoint function in cell cycle progression. We observed that knockdown of DRG2 in HeLa cells affected growth in a wound-healing assay, and tumorigenicity in nude mice xenografts. Flow cytometry assays and [(3)H] incorporation assays indicated that G2/M phase arrest was responsible for the decreased proliferation of these cells. Knockdown of DRG2 elicited down-regulation of the major mitotic promoting factor, the cyclin B1/Cdk1 complex, but up-regulation of the cell cycle arresting proteins, Wee1, Myt1, and p21. These findings identify a novel role of DRG2 in G2/M progression.
发育调控型GTP结合蛋白2（DRG2）在细胞生长中发挥重要作用。在此，我们探讨了DRG2与细胞周期进程中G2/M期检查点功能之间的联系。我们观察到，在HeLa细胞中敲低DRG2会影响伤口愈合实验中的细胞生长以及裸鼠异种移植瘤的致瘤性。流式细胞术分析和[³H]掺入分析表明，G2/M期阻滞是这些细胞增殖减少的原因。敲低DRG2导致主要的有丝分裂促进因子细胞周期蛋白B1/Cdk1复合物表达下调，但细胞周期阻滞蛋白Wee1、Myt1和p21表达上调。这些发现确定了DRG2在G2/M期进程中的新作用。
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