Yamamoto S, Nitta N, Kamiyama Y, Yamaoka Y, Ozawa K
Department of Surgery, Kyoto University Faculty of Medicine, Japan.
Eur Surg Res. 1989;21(2):98-104. doi: 10.1159/000129009.
To examine the effects of portal blood deprivation on energy metabolism of the liver, we studied: (1) the ketone body ratio (acetoacetate/beta-hydroxybutyrate) in liver tissue, which is in equilibrium with the free NAD+/NADH ratio in liver mitochondria, in the ligated lobe (LL) and nonligated lobe (NLL), (2) the hepatic energy charge [EC = (ATP + 1/2 ADP)/(ATP + ADP + AMP)] in both LL and NLL, and (3) the arterial blood ketone body ratio (BKBR) after left portal vein branch ligation (PBL) in rabbits. As found in LL after PBL, portal blood deprivation decreased the tissue ketone body ratio. The EC in LL significantly decreased after PBL, but recovered 7 days after PBL since the LL became atrophic. The BKBR remained within the normal range, even when 60% of the total liver was deprived of portal blood.
为研究门静脉血流阻断对肝脏能量代谢的影响，我们进行了以下研究：(1) 测定结扎叶 (LL) 和非结扎叶 (NLL) 肝组织中的酮体比率（乙酰乙酸/β-羟丁酸），该比率与肝线粒体中的游离NAD⁺/NADH比率处于平衡状态；(2) 测定LL和NLL中的肝脏能量负荷[EC = (ATP + 1/2 ADP)/(ATP + ADP + AMP)]；(3) 测定兔左门静脉分支结扎 (PBL) 后的动脉血酮体比率 (BKBR)。正如PBL后在LL中所发现的，门静脉血流阻断降低了组织酮体比率。PBL后LL中的EC显著降低，但在PBL 7天后恢复，因为LL发生了萎缩。即使全肝的60%被阻断门静脉血流，BKBR仍保持在正常范围内。
