Tanaka J, Ozawa K, Tobe T
Gastroenterology. 1979 Apr;76(4):691-6.
Changes in the free NAD+/NADH ratio and the energy charge (ATP + 1/2 ADP/ATP + ADP + AMP) of the liver were compared with the ratio of acetoacetate to beta-hydroxybutyrate of arterial blood in rabbits subjected to ligation of the common bile duct. Both the acetoacetate/beta-hydroxybutyrate ratio of the liver, which reflects mitochondrial free NAD+/NADH ratio, and the heptic energy charge decreased in accordance with the decrease of mitochondrial phosphorylative activity after the ligation. The decrease in the acetoacetate/beta-hydroxybutyrate ratio of the liver was attributed to a restricted mitochondrial reoxidation of NADH due to an inhibition of oxidative phosphorylation. Moreover, changes in the ratio of acetoacetate to beta-hydroxybutyrate in arterial blood were positively correlated with those of the liver (r = 0.695, P less than 0.01) and the hepatic energy charge (r = 0.844, P less than 0.01). It is suggested that the ratio of acetoacetate to beta-hydroxybutyrate in blood can reflect the energy charge of the liver in jaundiced rabbits.
将胆总管结扎的家兔肝脏中游离NAD⁺/NADH比值和能量负荷（ATP + 1/2 ADP/ATP + ADP + AMP）的变化与动脉血中乙酰乙酸与β-羟基丁酸的比值进行比较。反映线粒体游离NAD⁺/NADH比值的肝脏乙酰乙酸/β-羟基丁酸比值以及肝脏能量负荷，随着结扎后线粒体磷酸化活性的降低而降低。肝脏乙酰乙酸/β-羟基丁酸比值的降低归因于氧化磷酸化受抑制导致线粒体对NADH的再氧化受限。此外，动脉血中乙酰乙酸与β-羟基丁酸比值的变化与肝脏的变化呈正相关（r = 0.695，P < 0.01），与肝脏能量负荷也呈正相关（r = 0.844，P < 0.01）。提示血中乙酰乙酸与β-羟基丁酸的比值可反映黄疸家兔肝脏的能量负荷。
