Mesali Hamideh, Ajami Abolghasem, Hussein-Nattaj Hadi, Rafiei Alireza, Rajabian Zeinab, Asgarian-Omran Hossein, Hosseini Vahid, Taghvaei Tarang, Tehrani Mohsen
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Iran J Immunol. 2016 Sep;13(3):167-77.
Regulatory T Cells (Tregs) and Myeloid-Derived Suppressor Cells (MDSCs) are two main regulatory cells modulating the immune responses in inflammation and cancer.
To investigate and compare Tregs and MDSCs in peptic ulcer and gastric cancer.
Patients with dyspepsia were selected and divided into three groups of non-ulcer dyspepsia (NUD, n=22), peptic ulcer disease (PUD, n=25), and gastric cancer (GC, n=27) according to their endoscopic and histopathological examinations. Helicobacter pylori infection was diagnosed by rapid urease test and histopathology. The number of peripheral blood CD4+CD25+FoxP3+Tregs and CD14+HLA-DR- MDSCs were determined in all patients, by flow cytometry. The number of FoxP3+ regulatory T cells was also determined by immunohistochemistry (IHC).
The percentage of peripheral blood Treg cells in both PUD (0.81 ± 0.39, p<0.001) and GC groups (0.98 ± 0.65, p<0.001) were significantly higher than in NUD group (0.46 ± 0.10). These results were also confirmed by IHC. A significantly higher percentage of MDSCs in patients with PUD (0.73 ± 0.19, p<0.001) and GC (0.73 ± 0.16, p<0.001) was also observed when compared to NUD group (0.46 ± 0.16). There was no difference in the percentages of these two cell types between the PUD and GC groups. The percentages of Tregs and MDSCs in patients with PUD and GC were not significantly correlated.
Both Tregs and MDSCs showed higher frequencies in PUD and GC. These results suggest that immune-modulation by the Tregs and MDSCs may play a role in the pathogenesis of PUD and GC.
调节性T细胞（Tregs）和髓源性抑制细胞（MDSCs）是在炎症和癌症中调节免疫反应的两种主要调节细胞。
研究并比较消化性溃疡和胃癌中的Tregs和MDSCs。
选取消化不良患者，根据内镜和组织病理学检查分为非溃疡性消化不良组（NUD，n = 22）、消化性溃疡病组（PUD，n = 25）和胃癌组（GC，n = 27）。通过快速尿素酶试验和组织病理学诊断幽门螺杆菌感染。采用流式细胞术测定所有患者外周血CD4 + CD25 + FoxP3 + Tregs和CD14 + HLA - DR - MDSCs的数量。还通过免疫组织化学（IHC）测定FoxP3 +调节性T细胞的数量。
PUD组（0.81±0.39，p<0.001）和GC组（0.98±0.65，p<0.001）外周血Treg细胞百分比均显著高于NUD组（0.46±0.10）。这些结果也得到了IHC的证实。与NUD组（0.46±0.16）相比，PUD患者（0.73±0.19，p<0.001）和GC患者（0.73±0.16，p<0.001）中MDSCs的百分比也显著更高。PUD组和GC组这两种细胞类型的百分比没有差异。PUD和GC患者中Tregs和MDSCs的百分比无显著相关性。
PUD和GC中Tregs和MDSCs的频率均较高。这些结果表明，Tregs和MDSCs的免疫调节可能在PUD和GC的发病机制中起作用。
