de Souza Débora Nunes, Carnieli Pedro, Macedo Carla Isabel, de Novaes Oliveira Rafael, de Carvalho Ruthner Batista Helena Beatriz, Rodrigues Adriana Candido, Pereira Patricia Mariano Cruz, Achkar Samira Maria, Vieira Luiz Fernando Pereira, Kawai Juliana Galera Castilho
Pasteur Institute, Av. Paulista 393, São Paulo, SP, 01311-000, Brazil.
Institute of Agricultural and Forest Defense of Espírito Santo, St. Desembargador José Fortunato 95, Vitoria, ES, 29066-070, Brazil.
Arch Virol. 2017 Jan;162(1):71-77. doi: 10.1007/s00705-016-3079-1. Epub 2016 Sep 26.
Cases of canine rabies continue to occur in North and Northeast Brazil, and the number of notifications of rabies cases in wild canids has increased as a result of the expansion of urban areas at the expense of areas with native vegetation. In light of this, we performed molecular characterization of rabies virus isolates from dogs and Cerdocyon thous from various states in North and Northeast Brazil. In all, 102 samples from dogs (n = 56) and Cerdocyon thous (n = 46) collected between 2006 and 2012 were used. The nucleotide sequences obtained for the N gene of rabies virus were analyzed, and phylogenetic analysis revealed the presence of two distinct genetic lineages, one associated with canids and one with bats, and, within the canid cluster, two distinct sublineages circulating among dogs and Cerdocyon thous. In addition, phylogenetic groups associated with geographic region and fourteen cases of interspecific infection were observed among the isolates from canids. Our findings show that analysis of rabies virus lineages isolated from reservoirs such as canids must be constantly evaluated because the mutation rate is high.
巴西北部和东北部仍有犬类狂犬病病例发生，由于城市扩张以牺牲原生植被区域为代价，野生犬科动物狂犬病病例的报告数量有所增加。鉴于此，我们对来自巴西北部和东北部各州的犬类及食蟹狐的狂犬病病毒分离株进行了分子特征分析。总共使用了2006年至2012年间采集的102份来自犬类（n = 56）和食蟹狐（n = 46）的样本。对狂犬病病毒N基因获得的核苷酸序列进行了分析，系统发育分析显示存在两个不同的遗传谱系，一个与犬科动物相关，一个与蝙蝠相关，并且在犬科动物集群中，有两个不同的亚谱系在犬类和食蟹狐之间传播。此外，在犬科动物分离株中观察到与地理区域相关的系统发育组以及14例种间感染病例。我们的研究结果表明，由于突变率很高，必须不断评估从犬科动物等宿主分离的狂犬病病毒谱系。
