Carnieli Pedro, Brandão Paulo Eduardo, Carrieri Maria Luisa, Castilho Juliana Galera, Macedo Carla Isabel, Machado Lindenberg M, Rangel Normélia, de Carvalho Rosangela Cavalcanti, de Carvalho Vania Alves, Montebello Lucia, Wada Marcelo, Kotait Ivanete
Instituto Pasteur de São Paulo, SP, Brazil.
Virus Res. 2006 Sep;120(1-2):113-20. doi: 10.1016/j.virusres.2006.02.007. Epub 2006 May 15.
Rabies in wild canids in Northeastern Brazil is frequent and has been reported for some time, with episodes of rabies transmission from these animals to humans also reported. In this study, we analyzed the antigenic and genetic profiles of the rabies virus nucleoprotein gene, isolated from 20 samples taken from domestic animals and wild canids located in the Northeastern region of Brazil. All viruses isolated from domestic animals (dogs and cats) belonged to the antigenic variant 2 (AgV2). Among the wild animal samples, only four were AgV2, and nine showed a divergent antigenic profile. Phylogenetic analysis revealed two Brazilian clusters. Cluster 1 (Brazilian domestic carnivore-related strains) showed two subclusters, called 1A and 1B, and cluster 2 (Brazilian wild canid-related strains) also showed two subclusters, called 2A and 2B. The majority of the samples with divergent antigenic strains segregated into subcluster 2A. The intracluster identity of cluster 1 was 95.6% and that of cluster 2, 92.4%. When clusters 1 and 2 were compared, an identity of 88.6% was found. The genetic analysis of wild canid samples performed in this study indicates that there are two distinct rabies cycles among canids in Brazil, one represented by domestic canids and the other by wild canids. This study shows that the virus samples isolated in Northeastern Brazil are region and species-specific.
巴西东北部野生犬科动物中的狂犬病很常见，且已有一段时间的报道，同时也有这些动物将狂犬病传播给人类的病例报告。在本研究中，我们分析了从巴西东北部地区的家畜和野生犬科动物采集的20份样本中分离出的狂犬病病毒核蛋白基因的抗原和基因图谱。从家畜（狗和猫）分离出的所有病毒都属于抗原变异体2（AgV2）。在野生动物样本中，只有4份是AgV2，9份显示出不同的抗原图谱。系统发育分析揭示了两个巴西簇。簇1（与巴西家养食肉动物相关的毒株）显示出两个亚簇，称为1A和1B，簇2（与巴西野生犬科动物相关的毒株）也显示出两个亚簇，称为2A和2B。大多数具有不同抗原毒株的样本归入亚簇2A。簇1的簇内同一性为95.6%，簇2的为92.4%。当比较簇1和簇2时，发现同一性为88.6%。本研究对野生犬科动物样本进行的基因分析表明，巴西犬科动物中存在两个不同的狂犬病传播周期，一个以家养犬科动物为代表，另一个以野生犬科动物为代表。这项研究表明，在巴西东北部分离的病毒样本具有地区和物种特异性。
