Dori I, Parnavelas J G
Department of Anatomy and Developmental Biology, University College London, U.K.
Exp Brain Res. 1989;76(2):417-23. doi: 10.1007/BF00247899.
The cholinergic innervation of the rat cerebral cortex was examined in pre- and postnatal life using immunohistochemistry with a monoclonal antibody directed against choline acetyltransferase (ChAT). Our observations show that there are two separate phases in the development of the cholinergic innervation of the rat neocortex. The first, a transient phase, occurs in the late stages of gestation and in the perinatal period. During this time, ChAT-labelled cells (neuroblasts, as well as immature pyramidal and non-pyramidal neurons) are present throughout the entire rostro-caudal extent of the primordial cortex. The fate of these cells, which are not visible shortly after birth, is unknown as is their functional role in the developing cortex. The second phase in the development of the cholinergic innervation begins in the middle of the second postnatal week. At this stage only a few faintly stained neurons and fibres appear in the cortex. Their numbers and staining intensity increase gradually until the fifth postnatal week when ChAT-labelled neurons and axonal arbours appear indistinguishable from their adult counterparts. The pattern of development observed in the second phase parallels closely that shown in a recent analysis of cortical ChAT activity during postnatal life.
利用针对胆碱乙酰转移酶（ChAT）的单克隆抗体进行免疫组织化学方法，对大鼠出生前和出生后的大脑皮质胆碱能神经支配进行了研究。我们的观察结果表明，大鼠新皮质胆碱能神经支配的发育存在两个不同阶段。第一个阶段是短暂阶段，发生在妊娠后期和围产期。在此期间，ChAT标记的细胞（成神经细胞以及未成熟的锥体细胞和非锥体细胞）存在于原始皮质的整个前后范围内。这些细胞在出生后不久就消失了，其命运未知，它们在发育中的皮质中的功能作用也不清楚。胆碱能神经支配发育的第二个阶段始于出生后第二周中期。在这个阶段，皮质中仅出现少数染色较淡的神经元和纤维。它们的数量和染色强度逐渐增加，直到出生后第五周，此时ChAT标记的神经元和轴突分支与成年时的情况难以区分。在第二个阶段观察到的发育模式与最近对出生后皮质ChAT活性分析中显示的模式非常相似。
