Waters R S, McCandlish C A, Cooper N G
Department of Anatomy and Neurobiology, University of Tennessee, College of Medicine, Memphis 38163.
Exp Brain Res. 1990;81(2):234-40. doi: 10.1007/BF00228112.
Development of the barrel field representation of the forelimb in the primary somatosensory cortex (SI) was studied in normal and deafferented neonatal rat pups by means of the peroxidase conjugated lectin peanut agglutinin (PNA), which most likely binds to radial glial cells within barrel boundaries. 1. Alterations in lectin binding were seen in animals sacrificed on postnatal day 8 (PND-8) if deafferentation took place on PND-1 (day of birth) through PND-6. 2. Deafferentation on PND-5 or on PND-6 had the least effect on lectin binding. In these animals, lectin binding was reduced, although the prospective representation was intact. 3. Deafferentation on PND-2, 3, and 4 had the greatest effect on lectin binding. In these animals, lectin binding was reduced and the prospective cortical representation was disrupted. 4. Deafferentation on PND-1 resulted in reduced lectin binding, however the prospective cortical representation was only slightly impaired compared to that in animals deafferented on PND-2, 3, and 4. 5. These results suggest that SI barrel field boundaries are important to plasticity and that a sensitive period for predevelopment of the forelimb barrels consists of postnatal days 1 through 6. Furthermore, the formation of normal SI barrel field boundaries requires an ongoing interaction between incoming afferents and radial glial cells.
通过过氧化物酶结合凝集素花生凝集素（PNA），研究了正常和去传入神经的新生大鼠幼崽初级体感皮层（SI）中前肢桶状区表征的发育情况，PNA很可能与桶状边界内的放射状胶质细胞结合。1. 如果在出生后第1天（PND-1，出生日）至PND-6期间进行去传入神经手术，在出生后第8天（PND-8）处死的动物中可观察到凝集素结合的改变。2. 在PND-5或PND-6进行去传入神经手术对凝集素结合的影响最小。在这些动物中，凝集素结合减少，尽管预期表征完整。3. 在PND-2、3和4进行去传入神经手术对凝集素结合的影响最大。在这些动物中，凝集素结合减少，预期的皮层表征被破坏。4. 在PND-1进行去传入神经手术导致凝集素结合减少，然而与在PND-2、3和4进行去传入神经手术的动物相比，预期的皮层表征仅略有受损。5. 这些结果表明，SI桶状区边界对可塑性很重要，前肢桶状区预发育的敏感期为出生后第1天至第6天。此外，正常SI桶状区边界的形成需要传入神经和放射状胶质细胞之间持续的相互作用。
