Gründling Angelika, Lee Vincent T
Section of Microbiology and MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London SW7 2AZ, UK
Department of Cell Biology and Molecular Genetics, University of Maryland at College Park, College Park, MD 20742, USA
Philos Trans R Soc Lond B Biol Sci. 2016 Nov 5;371(1707). doi: 10.1098/rstb.2015.0503.
Signalling nucleotides are key molecules that help bacteria to rapidly coordinate cellular pathways and adapt to changes in their environment. During the past 10 years, the nucleotide signalling field has seen much excitement, as several new signalling nucleotides have been discovered in both eukaryotic and bacterial cells. The fields have since advanced quickly, aided by the development of important tools such as the synthesis of modified nucleotides, which, combined with sensitive mass spectrometry methods, allowed for the rapid identification of specific receptor proteins along with other novel genome-wide screening methods. In this review, we describe the principle concepts of nucleotide signalling networks and summarize the recent work that led to the discovery of the novel signalling nucleotides. We also highlight current approaches applied to the research in the field as well as resources and methodological advances aiding in a rapid identification of nucleotide-specific receptor proteins.This article is part of the themed issue 'The new bacteriology'.
信号核苷酸是帮助细菌快速协调细胞通路并适应环境变化的关键分子。在过去十年中，核苷酸信号领域备受关注，因为在真核细胞和细菌细胞中都发现了几种新的信号核苷酸。此后，在重要工具的推动下，该领域迅速发展，这些工具包括修饰核苷酸的合成，它与灵敏的质谱方法相结合，能够快速鉴定特定的受体蛋白以及其他新的全基因组筛选方法。在这篇综述中，我们描述了核苷酸信号网络的基本概念，并总结了导致发现新型信号核苷酸的近期工作。我们还重点介绍了该领域研究中应用的当前方法，以及有助于快速鉴定核苷酸特异性受体蛋白的资源和方法学进展。本文是主题为“新细菌学”的特刊的一部分。
