Sima Lei, Fan Bifa, Yan Longtao, Shui Yuan
National Pain Management and Research Center, China-Japan Friendship Hospital, Beijing, China.
Evid Based Complement Alternat Med. 2016;2016:8028474. doi: 10.1155/2016/8028474. Epub 2016 Sep 8.
Objective. To explore the efficacy of electroacupuncture treatment in cancer induced bone pain (CIBP) rat model with morphine tolerance and explore changes of calcitonin-gene related peptide (CGRP) expression in dorsal root ganglion (DRG). Methods. Forty SD rats were divided into five groups: sham, CIBP (B), CIBP + morphine (BM), CIBP + electroacupuncture (BE), and CIBP + morphine + electroacupuncture (BME). B, BM, BE, and BME groups were prepared CIBP model. The latter three groups then accepted morphine, electroacupuncture, and morphine combined electroacupuncture, separately, nine days consecutively (M1 to M9). Mechanical withdraw threshold (MWT) was evaluated. Results. BE group only had differences in M1, M2, and M3 compared to B group (P < 0.01). From M5, BM group showed significantly decreased MWT. Electroacupuncture could obtain analgesic effects only at early stage (M1 to M5). From M5 to M9, BME had the differences with BM group (P < 0.01). IOD value of CGRP in BM and BME was substantially less than in B group. CGRP in BME was significantly lower than that in BM group (P < 0.01). Conclusion. When used in combination with electroacupuncture, morphine could result in improving analgesic effects and reducing tolerance. CGRP may be associated with pain behaviors.
目的。探讨电针治疗对吗啡耐受的癌性骨痛（CIBP）大鼠模型的疗效，并探讨背根神经节（DRG）中降钙素基因相关肽（CGRP）表达的变化。方法。将40只SD大鼠分为五组：假手术组、CIBP组（B组）、CIBP + 吗啡组（BM组）、CIBP + 电针组（BE组）和CIBP + 吗啡 + 电针组（BME组）。B组、BM组、BE组和BME组制备CIBP模型。后三组分别连续九天接受吗啡、电针以及吗啡联合电针治疗（M1至M9）。评估机械缩足阈值（MWT）。结果。与B组相比，BE组仅在M1、M2和M3时有差异（P < 0.01）。从M5开始，BM组MWT显著降低。电针仅在早期（M1至M5）有镇痛效果。从M5至M9，BME组与BM组有差异（P < 0.01）。BM组和BME组中CGRP的积分光密度（IOD）值显著低于B组。BME组中CGRP显著低于BM组（P < 0.01）。结论。吗啡与电针联合使用可提高镇痛效果并降低耐受性。CGRP可能与疼痛行为有关。
