Kunprom Chonticha, Pramual Pairot
a Department of Biology, Faculty of Science, Mahasarakham University, Kantharawichai District, Maha Sarakham 44150 Thailand.
b Biodiversity and Traditional Knowledge Research Unit, Faculty of Science, Mahasarakham University, Kantharawichai District, Maha Sarakham 44150 Thailand.
Genome. 2016 Oct;59(10):792-804. doi: 10.1139/gen-2015-0110. Epub 2016 May 3.
The objectives of this study were to examine the genetic variation in fruit flies (Diptera: Tephritidae) in Thailand and to test the efficiency of the mitochondrial cytochrome c oxidase subunit I (COI) barcoding region for species-level identification. Twelve fruit fly species were collected from 24 host plant species of 13 families. The number of host plant species for each fruit fly species ranged between 1 and 11, with Bactrocera correcta found in the most diverse host plants. A total of 123 COI sequences were obtained from these fruit fly species. Sequences from the NCBI database were also included, for a total of 17 species analyzed. DNA barcoding identification analysis based on the best close match method revealed a good performance, with 94.4% of specimens correctly identified. However, many specimens (3.6%) had ambiguous identification, mostly due to intra- and interspecific overlap between members of the B. dorsalis complex. A phylogenetic tree based on the mitochondrial barcode sequences indicated that all species, except for the members of the B. dorsalis complex, were monophyletic with strong support. Our work supports recent calls for synonymization of these species. Divergent lineages were observed within B. correcta and B. tuberculata, and this suggested that these species need further taxonomic reexamination.
本研究的目的是检测泰国果蝇（双翅目：实蝇科）的遗传变异，并测试线粒体细胞色素c氧化酶亚基I（COI）条形码区域在物种水平鉴定中的效率。从13个科的24种寄主植物中收集了12种果蝇。每种果蝇的寄主植物种类数量在1到11种之间，其中芒果果肉蝇（Bactrocera correcta）的寄主植物种类最为多样。从这些果蝇物种中总共获得了123条COI序列。还纳入了来自NCBI数据库的序列，总共分析了17个物种。基于最佳匹配方法的DNA条形码鉴定分析显示效果良好，94.4%的标本被正确鉴定。然而，许多标本（3.6%）的鉴定结果不明确，主要是由于桔小实蝇复合种（B. dorsalis complex）成员之间的种内和种间重叠。基于线粒体条形码序列的系统发育树表明，除桔小实蝇复合种的成员外，所有物种均为单系且支持度很高。我们的工作支持了最近对这些物种进行同义词化的呼吁。在芒果果肉蝇和瘤背果实蝇（B. tuberculata）中观察到了不同的谱系，这表明这些物种需要进一步进行分类学重新审视。
