Kunprom Chonticha, Pramual Pairot
Department of Biology, Faculty of Science, Mahasarkham University, Kantharawichai District, Thailand.
Mitochondrial DNA A DNA Mapp Seq Anal. 2019 Dec;30(8):861-873. doi: 10.1080/24701394.2019.1693550. Epub 2019 Nov 21.
Fruit flies (Diptera: Tephritidae) are significant insect pests of many commercially important fruits and vegetables. Therefore, rapid and accurate species identification methods are required for the regulation, management and quarantine of these pests. In this study, we examined the efficiency of mitochondrial cytochrome oxidase I sequences for species identification of fruit flies in Thailand. Data analyses based on 42 fruit fly taxa revealed moderate performance of this genetic marker. There were 14 taxa that have no barcode gap and thus could not be identified unambiguously to species by this methodology. Taxonomic uncertainty, inadequate variation of the marker and misidentifications of specimens deposited in the public database are the most likely factors explaining unsuccessful identification. DNA barcodes also revealed cryptic diversity in five taxa (, , , , ). These species require further taxonomic investigation of if they are different cryptic taxa or are indications of geographic structuring of within single species.
果蝇（双翅目：实蝇科）是许多具有重要商业价值的水果和蔬菜的重要害虫。因此，对于这些害虫的监管、管理和检疫，需要快速准确的物种鉴定方法。在本研究中，我们检验了线粒体细胞色素氧化酶I序列用于泰国果蝇物种鉴定的效率。基于42个果蝇分类单元的数据分析显示该遗传标记的性能一般。有14个分类单元没有条形码间隙，因此无法通过这种方法明确鉴定到物种。分类学上的不确定性、标记变异不足以及公共数据库中保存标本的错误鉴定是解释鉴定失败的最可能因素。DNA条形码还揭示了五个分类单元（， ， ， ， ）中的隐存多样性。这些物种是否为不同的隐存分类单元或是否表明单一物种内的地理结构，需要进一步进行分类学研究。
