使用去细胞猪心包构建屈肌腱鞘
Flexor Tendon Sheath Engineering Using Decellularized Porcine Pericardium.
作者信息
Megerle Kai, Woon Colin, Kraus Armin, Raghavan Shyam, Pham Hung, Chang James
机构信息
Palo Alto, Calif.
From the Division of Plastic and Reconstructive Surgery, Stanford University Medical Center; and the Section of Plastic Surgery, VA Palo Alto Health Care System.
出版信息
Plast Reconstr Surg. 2016 Oct;138(4):630e-641e. doi: 10.1097/PRS.0000000000002459.
BACKGROUND
The flexor tendon sheath is an ideal target for tissue engineering because it is difficult to reconstruct by conventional surgical methods. The authors hypothesized that decellularized porcine pericardium can be used as a scaffold for engineering a biologically active tendon sheath.
METHODS
The authors' protocol removed cellular material from the pericardium and preserved the structural architecture in addition to the collagen and glycosaminoglycan content. The scaffold was successfully reseeded with human sheath synoviocytes and human adipose-derived stem cells. Cells were evaluated for 8 weeks after reseeding.
RESULTS
The reseeded construct demonstrated continuous production of hyaluronic acid, the main component of synovial fluid. After being seeded on the membrane, adipose-derived stem cells demonstrated down-regulation of collagen I and III and up-regulation of hyaluronan synthase 2.
CONCLUSION
The results indicate that decellularized porcine pericardium may be a potential scaffold for engineering a biologically active human tendon sheath.
背景
屈肌腱腱鞘是组织工程的理想靶点，因为传统手术方法难以对其进行重建。作者推测，脱细胞猪心包可作为构建生物活性腱鞘的支架材料。
方法
作者的方案去除了心包中的细胞物质，除保留了胶原和糖胺聚糖成分外，还保留了其结构框架。该支架成功地接种了人腱鞘滑膜细胞和人脂肪来源干细胞。接种后对细胞进行了8周的评估。
结果
重新接种的构建体显示出持续产生透明质酸，这是滑液的主要成分。脂肪来源干细胞接种到膜上后，I型和III型胶原表达下调，透明质酸合酶2表达上调。
结论
结果表明，脱细胞猪心包可能是构建生物活性人腱鞘的潜在支架材料。
Zotero 插件