Faculty of Veterinary Medicine, Veterinary Teaching Hospital, Department for Horses, University of Leipzig, D 04103 Leipzig, Germany.
Saxonian Incubator for Clinical Translation, University of Leipzig, D-04103 Leipzig, Germany.
Int J Mol Sci. 2019 Nov 3;20(21):5474. doi: 10.3390/ijms20215474.
Transforming growth factor beta 3 (TGFβ3) promotes tenogenic differentiation and may enhance tendon regeneration in vivo. This study aimed to apply TGFβ3 absorbed in decellularized equine superficial digital flexor tendon scaffolds, and to investigate the bioactivity of scaffold-associated TGFβ3 in an in vitro model. TGFβ3 could effectively be loaded onto tendon scaffolds so that at least 88% of the applied TGFβ3 were not detected in the rinsing fluid of the TGFβ3-loaded scaffolds. Equine adipose tissue-derived multipotent mesenchymal stromal cells (MSC) were then seeded on scaffolds loaded with 300 ng TGFβ3 to assess its bioactivity. Both scaffold-associated TGFβ3 and TGFβ3 dissolved in the cell culture medium, the latter serving as control group, promoted elongation of cell shapes and scaffold contraction ( < 0.05). Furthermore, scaffold-associated and dissolved TGFβ3 affected MSC musculoskeletal gene expression in a similar manner, with an upregulation of tenascin c and downregulation of other matrix molecules, most markedly decorin ( < 0.05). These results demonstrate that the bioactivity of scaffold-associated TGFβ3 is preserved, thus TGFβ3 application via absorption in decellularized tendon scaffolds is a feasible approach.
转化生长因子β3（TGFβ3）可促进腱细胞分化，并可能增强体内肌腱再生。本研究旨在将 TGFβ3 吸附于去细胞化的马腕屈肌腱支架中，并在体外模型中研究支架相关 TGFβ3 的生物活性。TGFβ3 可有效加载到肌腱支架上，以至于 TGFβ3 加载支架的冲洗液中检测不到至少 88%的 TGFβ3。随后将马脂肪组织来源的多能间充质基质细胞（MSC）接种到负载 300ng TGFβ3 的支架上，以评估其生物活性。支架相关 TGFβ3 和溶解在细胞培养基中的 TGFβ3（后者作为对照组）均促进细胞形态的伸长和支架的收缩（<0.05）。此外，支架相关和溶解的 TGFβ3 以相似的方式影响 MSC 肌肉骨骼基因表达，其中腱蛋白 C 的上调和其他基质分子的下调最为明显（<0.05）。这些结果表明，支架相关 TGFβ3 的生物活性得以保留，因此通过吸收于去细胞化肌腱支架来应用 TGFβ3 是一种可行的方法。
