Oluyori Abimbola Peter, Shaw Arun Kumar, Olatunji Gabriel Ademola, Rastogi Preeti, Meena Sanjeev, Datta Dipak, Arora Ashish, Reddy Sammajay, Puli Saidha
a Department of Physical Sciences , Landmark University , Omuaran , Kwara State , Nigeria.
b Medicinal and Process Chemistry Division , Central Drug Research Institute , Lucknow , India.
Nutr Cancer. 2016 Nov-Dec;68(8):1330-1337. doi: 10.1080/01635581.2016.1225107. Epub 2016 Sep 27.
A bioassay-guided fractionation of an alcoholic extract from the peels of Ipomoea batatas Lam has been carried out. Sulforhodamine B and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays were used to evaluate the anticancer and antioxidant potential, respectively, while silica gel column chromatography (CC) was used to isolate compounds that were characterized using 1D- and 2D-NMR (Nuclear Magnetic Resonance) and mass spectrometry. The alcoholic extract was fractionated into n-hexane, ethyl acetate, n-butanol, and water. The n-hexane fraction which showed the most promising anticancer activity was further fractionated via silica gel CC into IB-F002A, IB-F002B, and IB-F002C. Of these, IB-F002C was the most active with IC values 24.75, 47.91, 52.37, 34.17, 46.07, and 25.89 μg/ml against breast, colon-1, colon-2, ovary, lung, and head/neck cancer cell lines, respectively. The bioassay-guided isolation from IB-F002C afforded a glucocerebroside, which showed 10.51%, 12.19%, 16.14%, and 34.05% inhibition of head and neck, breast-1, colon-1, and ovarian cancer cell lines, respectively. Octadecyl coumarate, 7-hydroxycoumarin, and 6-methoxy-7-hydroxycoumarin that showed different antioxidant potentials were also identified in this study. Sweet potato peel, which is usually discarded as waste, contains constituents that can serve as dietary components to prevent the development of different types of cancer.
对甘薯（Ipomoea batatas Lam）果皮乙醇提取物进行了生物活性导向的分离。分别采用磺酰罗丹明B和2,2-二苯基-1-苦基肼（DPPH）法评估其抗癌和抗氧化潜力，同时使用硅胶柱色谱法（CC）分离化合物，并通过一维和二维核磁共振（NMR）以及质谱对其进行表征。乙醇提取物被分离成正己烷、乙酸乙酯、正丁醇和水相。显示出最有前景抗癌活性的正己烷馏分通过硅胶柱色谱进一步分离为IB-F002A、IB-F002B和IB-F002C。其中，IB-F002C活性最强，对乳腺癌、结肠癌细胞系1、结肠癌细胞系2、卵巢癌、肺癌和头颈癌细胞系的IC值分别为24.75、47.91、52.37、34.17、46.07和25.89μg/ml。从IB-F002C中通过生物活性导向分离得到一种葡萄糖脑苷脂，其对头颈癌、乳腺癌细胞系1、结肠癌细胞系1和卵巢癌细胞系的抑制率分别为10.51%、12.19%、16.14%和34.05%。本研究还鉴定出了具有不同抗氧化潜力的十八烷基香豆酸酯、7-羟基香豆素和6-甲氧基-7-羟基香豆素。通常作为废弃物丢弃的甘薯皮含有可作为膳食成分预防不同类型癌症发生的成分。
