催产素和中催产素在两种澳大利亚有袋动物——长吻袋狸（Isoodon macrourus）和帚尾袋貂（Trichosurus vulpecula）下丘脑内的免疫细胞化学定位。
Immunocytochemical location of oxytocin and mesotocin within the hypothalamus of two Australian marsupials, the bandicoot Isoodon macrourus and the brushtail possum Trichosurus vulpecula.
作者信息
Gemmell R T, Sernia C
机构信息
Department of Anatomy, University of Queensland, St. Lucia, Brisbane, Australia.
出版信息
Gen Comp Endocrinol. 1989 Jul;75(1):96-102. doi: 10.1016/0016-6480(89)90013-0.
The presence of oxytocin and mesotocin in the hypothalamus of two Australian marsupials, the bandicoot (Isoodon macrourus) and the brushtail possum (Trichosurus vulpecula), was examined by immunocytochemistry. Tissue was fixed in paraformaldehyde in phosphate buffer and immunoreactive cells were detected using highly specific rabbit antioxytocin and sheep anti-mesotocin as primary antisera. Immunoreactive oxytocin cells were demonstrated in the paraventricular and supraoptic nuclei of bandicoot and possum hypothalami, with greater density being observed in paraventricular nuclei. Immunoreactive mesotocin cells were also found in both hypothalamic nuclei of the possum but not of the bandicoot. The same cells appeared to stain for both peptides.
通过免疫细胞化学方法，检测了两种澳大利亚有袋动物——袋狸（长鼻袋狸）和帚尾袋貂下丘脑内催产素和中催产素的存在情况。组织用磷酸盐缓冲液中的多聚甲醛固定，使用高度特异性的兔抗催产素和羊抗中催产素作为一抗血清来检测免疫反应性细胞。在袋狸和袋貂下丘脑的室旁核和视上核中均发现了免疫反应性催产素细胞，室旁核中的密度更高。在袋貂的两个下丘脑核中也发现了免疫反应性中催产素细胞，但在袋狸中未发现。同一细胞似乎对两种肽均呈阳性染色。
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