Dusbábek F, Lukes S, Matha V, Grubhoffer L
Institute of Parasitology and Institute of Entomology, Czechoslovak Academy of Sciences, Ceské Budĕjovice.
Folia Parasitol (Praha). 1989;36(1):83-92.
Circulating antibodies to larval Argas polonicus antigen detected in the blood of pigeons by means of ELISA reach their highest level 3-6 days post-tick attachment. During 6-8 days post infestation when most larvae detach from their host, there is an abrupt drop of the antibody level in blood followed by second peak at day 10-15. During the secondary and subsequent infestations the dynamics of the antibody production is analogous, but the maximum absorbance values found are higher with each following infestation. This is in direct correlation with the growth of immune resistance of hosts. The transfer of immunoglobulins of resistant pigeons produces in naive hosts a partial resistance in a statistically significant (P less than 0.01) reduction of the number of engorged larvae, in the shortening of larval feeding period and in the decrease of their mean weight after feeding. However this resistance was significantly (P less than 0.01) less expressed than in naturally resistant pigeons during secondary infestation. The protracted effect on the duration of premoulting period and the percentage of moulted larvae manifested in larvae after secondary infestation was not apparent in molecular weight of approximately 19, 21, 23, 27, 45 and 165 kilodaltons, were recognized by serum of resistant pigeons.
通过酶联免疫吸附测定法在鸽子血液中检测到的针对波兰锐缘蜱幼虫抗原的循环抗体，在蜱附着后3 - 6天达到最高水平。在感染后6 - 8天，当大多数幼虫从宿主身上脱落时，血液中的抗体水平会突然下降，随后在第10 - 15天出现第二个峰值。在二次及后续感染期间，抗体产生的动态情况类似，但每次后续感染时测得的最大吸光度值更高。这与宿主免疫抗性的增强直接相关。抗性鸽子的免疫球蛋白转移到未感染的宿主中，会使未感染宿主产生部分抗性，表现为饱血幼虫数量在统计学上显著减少（P小于0.01）、幼虫取食期缩短以及取食后平均体重下降。然而，在二次感染期间，这种抗性的表现明显低于自然抗性鸽子（P小于0.01）。二次感染后幼虫对蜕皮前期持续时间和蜕皮幼虫百分比的长期影响，在分子量约为19、21、23、27、45和165千道尔顿的情况下并不明显，这些分子量的物质可被抗性鸽子的血清识别。
