Perälä M, Hänninen M, Hästbacka J, Elima K, Vuorio E
Department of Medical Biochemistry, University of Turku, Finland.
FEBS Lett. 1993 Mar 15;319(1-2):177-80. doi: 10.1016/0014-5793(93)80062-y.
Type IX collagen, a heterotrimer of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chains, is a cartilage-specific fibril-associated collagen. In the process of characterizing genomic clones for the mouse alpha 2(IX) collagen gene four pairs of oligonucleotide primers designed for amplification of murine exon sequences were also utilized to construct cDNA clones for human alpha 2(IX) collagen spanning > 90% of the coding region. The amino acid and nucleotide sequence identities between human and chick are 78% and 71%, respectively. Localization of the COL9A2 gene to human chromosome 1 was subsequently performed using a panel of DNAs from human/rodent somatic cell hybrids.
IX型胶原蛋白是由α1(IX)、α2(IX)和α3(IX)链组成的异源三聚体，是一种软骨特异性的原纤维相关胶原蛋白。在对小鼠α2(IX)胶原蛋白基因的基因组克隆进行表征的过程中，为扩增小鼠外显子序列而设计的四对寡核苷酸引物也被用于构建跨越人类α2(IX)胶原蛋白编码区90%以上的cDNA克隆。人类和鸡之间的氨基酸和核苷酸序列同一性分别为78%和71%。随后使用一组来自人/啮齿动物体细胞杂种的DNA将COL9A2基因定位到人类染色体1上。
