Ghafouri Syed Ali, Langeroudi Arash Ghalyanchi, Maghsoudloo Hossein, Tehrani Farshad, Khaltabadifarahani Reza, Abdollahi Hamed, Fallah Mohammad Hossein
Iranian Veterinary Organization, Tehran, Iran.
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Virus Genes. 2017 Feb;53(1):117-120. doi: 10.1007/s11262-016-1394-y. Epub 2016 Sep 27.
Highly pathogenic avian influenza (HPAI) viruses of the H5N1 subtype have been diversified into multiple phylogenetic clades over the past decade and are highly genetically variable. In June 2015, one outbreak of HPAI H5N1 in backyard chickens was reported in the Nogardan village of the Mazandaran Province. Tracheal tissues were taken from the dead domestic chickens (n = 10) and processed for RT-PCR. The positive samples (n = 10) were characterized as HPAI H5N1 by sequencing analysis for the hemagglutinin and neuraminidase genes. Phylogenetic analysis of the samples revealed that the viruses belonged to clade 2.3.2.1c, and cluster with the HPAI H5N1 viruses isolated from different avian species in Bulgaria, Romania, and Nigeria in 2015. They were not closely related to other H5N1 isolates detected in previous years in Iran. Our study provides new insights into the evolution and genesis of H5N1 influenza in Iran and has important implications for targeting surveillance efforts to rapidly identify the spread of the virus into and within Iran.
在过去十年中，H5N1亚型高致病性禽流感（HPAI）病毒已分化为多个系统发育分支，并且具有高度的遗传变异性。2015年6月，伊朗马赞德兰省诺加尔丹村报告了一起后院鸡群感染HPAI H5N1的疫情。从死亡的家鸡（n = 10）身上采集气管组织并进行逆转录聚合酶链反应（RT-PCR）检测。通过对血凝素和神经氨酸酶基因进行测序分析，将10份阳性样本鉴定为HPAI H5N1。对样本的系统发育分析表明，这些病毒属于2.3.2.1c分支，并且与2015年在保加利亚、罗马尼亚和尼日利亚从不同禽类中分离出的HPAI H5N1病毒聚集在一起。它们与伊朗前几年检测到的其他H5N1分离株没有密切关系。我们的研究为伊朗H5N1流感的进化和起源提供了新的见解，对于针对性监测工作以快速识别病毒在伊朗境内外的传播具有重要意义。
