Laboratório de Ictioparasitologia, Central de Laboratórios de Ciência e Tecnologia Ambiental, Pró-reitoria de Pesquisa e Pós-graduação, Universidade do Sagrado Coração (USC); Rua Irmã Arminda, 10-50, 17011-160 Bauru, São Paulo, Brazil.
Instituto de Biociências de Botucatu, Universidade Estadual Paulista "Julio de Mesquita Filho" (UNESP), Botucatu, São Paulo, Brazil.
Sci Total Environ. 2017 Jan 1;575:836-840. doi: 10.1016/j.scitotenv.2016.09.132. Epub 2016 Sep 25.
Pollution in aquatic ecosystems due to negative human activities remains a problem in both freshwater and marine environments and is an ongoing subject of research. Several studies have shown that some fish parasites can be used as a tool for biomonitoring because they demonstrate higher metal accumulation capacity compared to their host tissues. However, compared to acanthocephalans, information regarding the absorption mechanisms and accumulation rates in nematodes is relatively limited. Here, we evaluated the potential of larvae Contracaecum sp. (L3) as indicators of contamination by metals by analyzing thirteen element concentrations: magnesium (Mg), aluminum (Al), titanium (Ti), chromium (Cr), manganese (Mn), iron (Fe), nickel (Ni), copper (Cu), zinc (Zn), arsenic (As), cadmium (Cd), barium (Ba), and lead (Pb) in the parasites and host Acestrorhynchus lacustris, using inductively coupled plasma mass spectrometry. Twelve of the thirteen analyzed elements were detected in at least 2-fold higher concentrations (e.g. Ni) and were up to approximately 50-fold higher (e.g. Pb) in parasites than in host tissues, including elements known for their high toxicity (As, Cd, Pb) and those considered to be essential (Cu and Zn). Our results suggest that Contracaecum sp. larvae can be used as bioindicators of metal contamination because even in early stages of development, numerous essential and non-essential elements were accumulated, making this system a useful tool for monitoring polluted environments.
由于人类的负面活动，水生生态系统中的污染仍然是淡水和海洋环境中的一个问题，也是一个正在进行的研究课题。一些研究表明，一些鱼类寄生虫可以作为生物监测的工具，因为它们比宿主组织表现出更高的金属积累能力。然而，与棘头虫相比，关于线虫吸收机制和积累率的信息相对有限。在这里，我们评估了幼虫 Contracaecum sp.（L3）作为金属污染指示剂的潜力，通过分析 13 种元素的浓度：镁（Mg）、铝（Al）、钛（Ti）、铬（Cr）、锰（Mn）、铁（Fe）、镍（Ni）、铜（Cu）、锌（Zn）、砷（As）、镉（Cd）、钡（Ba）和铅（Pb）在寄生虫和宿主湖拟丽鱼体内，使用电感耦合等离子体质谱法。在寄生虫中检测到至少 13 种分析元素中的 12 种，其浓度是宿主组织的 2 倍以上（如 Ni），高达 50 倍以上（如 Pb），其中包括高毒性元素（As、Cd、Pb）和被认为是必需元素（Cu 和 Zn）。我们的研究结果表明，幼虫 Contracaecum sp.可以作为金属污染的生物指示剂，因为即使在发育的早期阶段，也积累了大量的必需和非必需元素，使这个系统成为监测污染环境的有用工具。
