Yan Xinlei, Tao Geru, Liu Xianyong, Ji Yongsheng, Suo Xun
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China; National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China; National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China; Key Laboratory of Zoonosis of Ministry of Agriculture & College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Exp Parasitol. 2016 Nov;170:193-197. doi: 10.1016/j.exppara.2016.09.005. Epub 2016 Sep 25.
Egress is a vital step in the endogenous development of apicomplexan parasites, as it assures the parasites exit from consumed host cells and entry into fresh ones. However, little information has previously been reported on this step of Eimeria spp. In this study, we investigated in vitro egress of Eimeria tenella sporozoites triggered by acetaldehyde. We found that addition of exogenous acetaldehyde induces egress of sporozoites from primary chicken kidney cells (PCKs) and stimulate secretion of E. tenella microneme 2 protein (EtMic 2). Moreover, by using cellular calcium inhibitors, we further proved that these processes were dependent on the intracellular calcium of the parasites. Our findings provide clues to the study of interaction between eimerian parasites and their hosts.
逸出是顶复门寄生虫内源性发育的关键步骤，因为它确保寄生虫从被消耗的宿主细胞中逸出并进入新的宿主细胞。然而，此前关于艾美耳属原虫这一步骤的报道很少。在本研究中，我们研究了乙醛触发的柔嫩艾美耳球虫子孢子的体外逸出。我们发现添加外源性乙醛可诱导子孢子从原代鸡肾细胞（PCK）中逸出，并刺激柔嫩艾美耳球虫微小膜体蛋白2（EtMic 2）的分泌。此外，通过使用细胞钙抑制剂，我们进一步证明这些过程依赖于寄生虫的细胞内钙。我们的研究结果为研究艾美耳属寄生虫与其宿主之间的相互作用提供了线索。
