Bouza-Mora Laura, Dolz Gaby, Solórzano-Morales Antony, Romero-Zuñiga Juan José, Salazar-Sánchez Lizbeth, Labruna Marcelo B, Aguiar Daniel M
Escuela de Medicina Veterinaria, Universidad Nacional, Campus Benjamín Nuñez, Barreal de Heredia, Heredia, Costa Rica.
Escuela de Medicina, Universidad de Costa Rica, San José, Costa Rica.
Ticks Tick Borne Dis. 2017 Jan;8(1):36-40. doi: 10.1016/j.ttbdis.2016.09.012. Epub 2016 Sep 23.
This study focuses on the detection and identification of DNA and antibodies to Ehrlichia spp. in samples of blood bank donors in Costa Rica using molecular and serological techniques. Presence of Ehrlichia canis was determined in 10 (3.6%) out of 280 blood samples using polymerase chain reaction (PCR) targeting the ehrlichial dsb conserved gene. Analysis of the ehrlichial trp36 polymorphic gene in these 10 samples revealed substantial polymorphism among the E. canis genotypes, including divergent tandem repeat sequences. Nucleotide sequences of dsb and trp36 amplicons revealed a novel genotype of E. canis in blood bank donors from Costa Rica. Indirect immunofluorescence assay (IFA) detected antibodies in 35 (35%) of 100 serum samples evaluated. Thirty samples showed low endpoint titers (64-256) to E. canis, whereas five sera yielded high endpoint titers (1024-8192); these five samples were also E. canis-PCR positive. These findings represent the first report of the presence of E. canis in humans in Central America.
本研究聚焦于利用分子和血清学技术，在哥斯达黎加血库供血者的血液样本中检测和鉴定埃立克体属的DNA及抗体。采用针对埃立克体dsb保守基因的聚合酶链反应（PCR），在280份血液样本中的10份（3.6%）中检测到犬埃立克体的存在。对这10份样本中埃立克体trp36多态性基因的分析显示，犬埃立克体基因型之间存在大量多态性，包括不同的串联重复序列。dsb和trp36扩增子的核苷酸序列揭示了来自哥斯达黎加血库供血者中的一种新型犬埃立克体基因型。间接免疫荧光法（IFA）在100份评估的血清样本中的35份（35%）中检测到抗体。30份样本对犬埃立克体显示出低终点滴度（64 - 256），而5份血清产生了高终点滴度（1024 - 8192）；这5份样本也是犬埃立克体PCR阳性。这些发现代表了中美洲人类中存在犬埃立克体的首次报道。
