Graduate College of Biomedical Sciences, Western University of Health Sciences, Pomona, California.
Affiliated Veterinary Specialists, Orange Park, Florida.
Am J Trop Med Hyg. 2018 Aug;99(2):297-302. doi: 10.4269/ajtmh.17-0776. Epub 2018 Jun 21.
spp. are emerging infectious pathogens, especially in the Americas. Although is primarily a parasite of dogs, polymerase chain reaction-confirmed human infections have been reported from Mexico, Venezuela, and Costa Rica. This study reports the presence of DNA in 13.7% of 205 dogs from urban areas in Peru and of those, five were analyzed for phylogenetic variation using the Tandem Repeat Protein 36 (TRP36) gene. The use of the TRP36 gene for such analysis was validated against 16S rRNA and heat shock protein genes using Shannon's entropy bioinformatic approach. When compared with other strains previously reported, three unique and novel strains were detected. In addition, the TRP36 amino acid tandem repeat sequences of the Peruvian strains share close similarity to an strain detected from four human blood bank samples in Costa Rica. This study reports for the first time domestic dogs infected with strains closely related to a zoonotic strain, which may be of public health concern as dogs can be chronically infected with this pathogen.
种是新发传染病病原体，尤其在美洲地区。虽然主要寄生于犬，但已在墨西哥、委内瑞拉和哥斯达黎加报道了聚合酶链反应（PCR）确诊的人类感染病例。本研究报告了在秘鲁城区 205 只犬中，有 13.7%的犬存在 DNA，其中 5 只使用串联重复蛋白 36（TRP36）基因进行了系统发育变异分析。使用 Shannon 熵生物信息学方法对 TRP36 基因进行了 16S rRNA 和热休克蛋白基因的验证。与其他先前报道的株相比，检测到了三种独特的新型株。此外，秘鲁株的 TRP36 氨基酸串联重复序列与从哥斯达黎加四个血库样本中检测到的一株 株具有密切的相似性。本研究首次报告了感染与人畜共患病株密切相关的犬种，这可能引起公共卫生关注，因为犬可能长期感染这种病原体。
