化妆品中防腐剂的测定。II. 高效液相色谱法鉴别

Determination of preservatives in cosmetic products. II. High-performance liquid chromatographic identification.

作者信息

De Kruijf N, Schouten A, Rijk M A, Pranoto-Soetardhi L A

机构信息

TNO-CIVO Food Analysis Institute, Department of Toxicological Analysis, AJ Zeist, The Netherlands.

出版信息

J Chromatogr. 1989 May 19;469:317-28. doi: 10.1016/s0021-9673(01)96466-0.

DOI:10.1016/s0021-9673(01)96466-0

PMID:2768376

Abstract

A high-performance liquid chromatographic (HPLC) procedure is presented for the separation and identification of preservatives that are listed in the current EEC Council Directive on cosmetic products or have been permitted in the past. The method consists of an extraction of acidified cosmetics with methanol, and separation of the extracts by HPLC. Using two isocratic and two gradient reversed-phase HPLC systems, 47 preservatives were characterized by their retention times. The preservatives in three commercial cosmetic products were tentatively identified by the procedure described. The HPLC procedure is suitable for confirmation of the presence of preservatives in cosmetic products as indicated by a previously reported thin-layer chromatographic procedure. In general this method will permit the routine detection of preservatives in cosmetics in an approximate concentration of 0.01% (w/w).

摘要

本文介绍了一种高效液相色谱（HPLC）方法，用于分离和鉴定当前欧盟理事会化妆品指令中列出的或过去已获批准的防腐剂。该方法包括用甲醇萃取酸化的化妆品，并通过HPLC分离提取物。使用两个等度和两个梯度反相HPLC系统，通过保留时间对47种防腐剂进行了表征。通过所述方法初步鉴定了三种商业化妆品中的防腐剂。HPLC方法适用于确认先前报道的薄层色谱法所表明的化妆品中防腐剂的存在。一般来说，该方法将允许常规检测化妆品中浓度约为0.01%（w/w）的防腐剂。