Moore K E, Stretton R J
J Chromatogr. 1978 Aug 21;156(1):211-7. doi: 10.1016/s0021-9673(00)83142-8.
A method is described using high voltage agar gel electrophoresis for the separation and microbiological detection of preservatives in pharmaceutical and cosmetic products. The positions of the preservatives were detected as zones of inhibition of a test organism within the nutrient agar gel and can be characterised by their migration, shape and presence of a halo. A wide range of preservatives commonly used in pharmaceutical and cosmetic preparations could be detected in low concentrations. The method however was not suitable for certain volatile preservatives and many primarily antifungal compounds could be detected above their use concentration.
本文描述了一种使用高压琼脂凝胶电泳分离和微生物检测药品和化妆品中防腐剂的方法。防腐剂的位置通过营养琼脂凝胶中测试微生物的抑菌区域来检测，并且可以通过它们的迁移、形状和晕圈的存在来表征。药品和化妆品制剂中常用的多种防腐剂能够在低浓度下被检测到。然而，该方法不适用于某些挥发性防腐剂，并且许多主要的抗真菌化合物在其使用浓度以上才能被检测到。
