Rossini Valerio, Radulovic Katarina, Riedel Christian U, Niess Jan Hendrik
APC Microbiome Institute, University College Cork.
Division of Gastroenterology and Hepatology, University Hospital Basel.
J Vis Exp. 2016 Sep 18(115):54421. doi: 10.3791/54421.
Inflammatory bowel disease (IBD) is a chronic inflammation which affects the gastrointestinal tract (GIT). One of the best ways to study the immunological mechanisms involved during the disease is the T cell transfer model of colitis. In this model, immunodeficient mice (RAG(-/-) recipients) are reconstituted with naive CD4(+) T cells from healthy wild type hosts. This model allows examination of the earliest immunological events leading to disease and chronic inflammation, when the gut inflammation perpetuates but does not depend on a defined antigen. To study the potential role of antigen presenting cells (APCs) in the disease process, it is helpful to have an antigen-driven disease model, in which a defined commensal-derived antigen leads to colitis. An antigen driven-colitis model has hence been developed. In this model OT-II CD4(+) T cells, that can recognize only specific epitopes in the OVA protein, are transferred into RAG(-/-) hosts challenged with CFP-OVA-expressing E. coli. This model allows the examination of interactions between APCs and T cells in the lamina propria.
炎症性肠病（IBD）是一种影响胃肠道（GIT）的慢性炎症。研究该疾病过程中涉及的免疫机制的最佳方法之一是结肠炎的T细胞转移模型。在这个模型中，免疫缺陷小鼠（RAG(-/-)受体）用来自健康野生型宿主的初始CD4(+) T细胞进行重建。当肠道炎症持续存在但不依赖于特定抗原时，这个模型可以检测导致疾病和慢性炎症的最早免疫事件。为了研究抗原呈递细胞（APC）在疾病过程中的潜在作用，拥有一个抗原驱动的疾病模型是有帮助的，在这个模型中，特定的共生菌衍生抗原会导致结肠炎。因此，已经开发了一种抗原驱动的结肠炎模型。在这个模型中，只能识别OVA蛋白中特定表位的OT-II CD4(+) T细胞被转移到用表达CFP-OVA的大肠杆菌攻击的RAG(-/-)宿主中。这个模型可以检测固有层中APC和T细胞之间的相互作用。
