Shahryari Varahram, Nip Hannah, Saini Sharanjot, Dar Altaf A, Yamamura Soichiro, Mitsui Yozo, Colden Melissa, Bucay Nathan, Tabatabai Laura Z, Greene Kirsten, Deng Guoren, Tanaka Yuichiro, Dahiya Rajvir, Majid Shahana
Department of Urology, VA Medical Center and UCSF.
California Pacific Medical Center Research Institute.
J Vis Exp. 2016 Aug 29(114):54125. doi: 10.3791/54125.
To study the multifaceted biology of prostate cancer, pre-clinical in vivo models offer a range of options to uncover critical biological information about this disease. The human orthotopic prostate cancer xenograft mouse model provides a useful alternative approach for understanding the specific interactions between genetically and molecularly altered tumor cells, their organ microenvironment, and for evaluation of efficacy of therapeutic regimens. This is a well characterized model designed to study the molecular events of primary tumor development and it recapitulates the early events in the metastatic cascade prior to embolism and entry of tumor cells into the circulation. Thus it allows elucidation of molecular mechanisms underlying the initial phase of metastatic disease. In addition, this model can annotate drug targets of clinical relevance and is a valuable tool to study prostate cancer progression. In this manuscript we describe a detailed procedure to establish a human orthotopic prostate cancer xenograft mouse model.
为了研究前列腺癌的多方面生物学特性，临床前体内模型提供了一系列选项，以揭示有关这种疾病的关键生物学信息。人原位前列腺癌异种移植小鼠模型为理解基因和分子改变的肿瘤细胞与其器官微环境之间的特定相互作用，以及评估治疗方案的疗效提供了一种有用的替代方法。这是一个特征明确的模型，旨在研究原发性肿瘤发展的分子事件，并且它概括了转移级联中在肿瘤细胞栓塞和进入循环之前的早期事件。因此，它有助于阐明转移性疾病初始阶段的分子机制。此外，该模型可以注释具有临床相关性的药物靶点，是研究前列腺癌进展的宝贵工具。在本手稿中，我们描述了建立人原位前列腺癌异种移植小鼠模型的详细步骤。
